SUPPRESSION OF LYTIC EFFECT OF BETA LACTAMS ON ESCHERICHIA-COLI AND OTHER BACTERIA
SUPPRESSION OF LYTIC EFFECT OF BETA LACTAMS ON ESCHERICHIA-COLI AND OTHER BACTERIA
复制标题
DOI：
10.1073/pnas.73.9.3293
复制
发表时间：
1976-01-01
影响因子：
11.1
通讯作者：
TOMASZ, A
中科院分区：
文献类型：
--
作者：
GOODELL, EW;LOPEZ, R;TOMASZ, A
E. pH 5的大肠杆菌保护细菌免受β-当细胞在pH 7或7.5下生长时通常观察到内酰胺抗生素，即，实验室实验中常规使用的pH值。β的典型效应是：在中性和酸性pH条件下生长的培养物中，内酰胺抗生素对细胞形状和伸长以及细胞分裂的影响似乎相似。肺炎球菌、葡萄球菌、链球菌和枯草芽孢杆菌也可表现出pH依赖性抗生素耐受性。pH依赖性抗生素耐受性的机制可能涉及产生更稳定的质膜或抑制催化抗生素诱导的裂解的胞壁蛋白水解酶的活性;这些酶分子的至少一部分可能定位于细胞表面并且可用于实验操作。
Growth of E. coli at pH 5 protected the bacteria against the lytic effect of .beta.-lactam antibiotics typically observed when the cells are grown at pH 7 or 7.5, i.e., the pH values routinely used in laboratory experiments. The typical effects of .beta.-lactam antibiotics on cellular shape and elongation and cell division appeared to be similar in cultures grown under neutral and acid pH conditions. The pH-dependent antibiotic tolerance can also be demonstrated with pneumococci, staphylococci, streptococci and Bacillus subtilis. The mechanism of the pH-dependent antibiotic tolerance may involve either the production of a more stable plasma membrane or the suppression of the activity of a murein hydrolase(s) that catalyzes the antibiotic-induced lysis; at least a fraction of these enzyme molecules may be localized at the cell surface and be accessible to experimental manipulation.