Evaluation of sample handling in relation to levels of tissue inhibitor of metalloproteinases-1 measured in blood by immunoassay
Evaluation of sample handling in relation to levels of tissue inhibitor of metalloproteinases-1 measured in blood by immunoassay
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DOI：
10.1177/172460080301800303
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发表时间：
2003-07-01
影响因子：
2
通讯作者：
Hogdall, EVS
中科院分区：
文献类型：
--
作者：
Holten-Andersen, MN;Schrohl, AS;Hogdall, EVS
背景资料：分析前的条件，如血液样品的制备和处理对TIMP-1水平的blood.Materials和方法的可能影响：收集血液在干燥管和管含有EDTA和保持在4 ℃或20 degreesC为1，4，8，24或72小时前处理成血清或EDTA血浆。此外，将血清和EDTA血浆样品冷冻和解冻1-8次。结果：处理时间长达72小时并没有显着影响血清中TIMP-1水平。在EDTA血浆中，TIMP-1水平在长达8小时内保持稳定;然而，如果样本保存24小时或更长时间，TIMP-1水平会增加（p
Background: The possible effect of preanalytical conditions such as blood sample preparation and handling on TIMP-1 levels in blood needs thorough investigation.Materials and methods: Blood was collected in dry tubes and tubes containing EDTA and kept at 4degreesC or 20degreesC for 1, 4, 8, 24 or 72 hours before processing into serum or EDTA plasma. in addition, serum and EDTA plasma samples were frozen and thawed 1-8 times. TIMP-1 was measured by ELISA.Results: Time to processing for up to 72 hours did not significantly affect TIMP-1 levels in serum. In EDTA plasma, TIMP-1 levels were stable for up to eight hours; however, if samples were kept for 24 hours or longer the TIMP-1 levels increased (p