Simulation of developmental changes in action potentials with ventricular cell models.
Simulation of developmental changes in action potentials with ventricular cell models.
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DOI：
10.1007/s11693-006-9002-4
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发表时间：
2007-03-01
期刊：
影响因子：
--
通讯作者：
Tomita, Masaru
中科院分区：
文献类型：
--
作者：
Itoh, Hitomi;Naito, Yasuhiro;Tomita, Masaru
在心肌细胞发育过程中，早期胚胎心室细胞表现出自发活动，在后期消失。动作电位的剧烈变化是由个体离子电流的发育变化介导的。因此，将单个离子电流重建成一个集成的数学模型将有助于更好地理解心肌细胞的发育。为了模拟啮齿动物心室细胞在三个代表性发育阶段的动作电位，离子电流、泵、交换器和肌浆网（SR）Ca（2+）动力学的定量变化表示为相对活性，乘以个别离子系统的电导或转换因子。早期胚胎心室细胞模型的模拟动作电位显示自发活动，在晚期胚胎和新生儿心室细胞模型的模拟动作电位中停止。与我们的模型的模拟能够再现与体外细胞的报告的特性一致的动作电位。不同发育阶段的啮齿动物心室细胞的动作电位可以用常见的数学方程组通过将离子电流、泵、交换剂和SR Ca（2+）动力学的电导或转换因子乘以相对活性来再现。
During cardiomyocyte development, early embryonic ventricular cells show spontaneous activity that disappears at a later stage. Dramatic changes in action potential are mediated by developmental changes in individual ionic currents. Hence, reconstruction of the individual ionic currents into an integrated mathematical model would lead to a better understanding of cardiomyocyte development. To simulate the action potential of the rodent ventricular cell at three representative developmental stages, quantitative changes in the ionic currents, pumps, exchangers, and sarcoplasmic reticulum (SR) Ca(2+) kinetics were represented as relative activities, which were multiplied by conductance or conversion factors for individual ionic systems. The simulated action potential of the early embryonic ventricular cell model exhibited spontaneous activity, which ceased in the simulated action potential of the late embryonic and neonatal ventricular cell models. The simulations with our models were able to reproduce action potentials that were consistent with the reported characteristics of the cells in vitro. The action potential of rodent ventricular cells at different developmental stages can be reproduced with common sets of mathematical equations by multiplying conductance or conversion factors for ionic currents, pumps, exchangers, and SR Ca(2+) kinetics by relative activities.