Vertical stratification of subsurface microbial community composition across geological formations at the Hanford Site
Vertical stratification of subsurface microbial community composition across geological formations at the Hanford Site
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DOI：
10.1111/j.1462-2920.2011.02659.x
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发表时间：
2012-02-01
影响因子：
5.1
通讯作者：
Konopka, Allan
中科院分区：
文献类型：
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作者：
Lin, Xueju;Kennedy, David;Konopka, Allan
对美国华盛顿州Richland附近Hanford Site 300地区9-52 m深度的21个样品进行了微生物多样性分析。在包括天然氧化还原过渡带的地质地层中分析了大约8000个近全长16 S rRNA基因序列。这些地层包括含氧粗粒汉福德组，细粒含氧和缺氧Ringold组沉积物，以及风化玄武岩组。我们分别检测到1233个和120个独特的细菌和古细菌OTU（97%同一性水平的操作分类单位）。微生物群落结构和丰富度在不同地质层中有很大差异。细菌OTU丰富度（Chao 1估计值）在上部Hanford地层中最高（> 700），并且在Hanford地层的底部下降到约120。就在林戈尔德氧化-缺氧界面上方，丰度约为325，在更深的还原带中下降到小于50。更深的Ringold地层的特点是优势（c。90%）的变形菌。在好氧沉积物中的细菌群落不仅包含了9个公认的门的成员，但也异常高比例的三个候选部门（GAL 15，NC 10和SPAM）。此外，13个新的系统发育顺序被确定内Deltaproteobacteria，一个分支丰富的微生物进行氧化还原转化的金属是重要的污染物在汉福德网站。
Microbial diversity in subsurface sediments at the Hanford Site 300 Area near Richland, Washington state (USA) was investigated by analysing 21 samples recovered from depths of 9-52 m. Approximately 8000 near full-length 16S rRNA gene sequences were analysed across geological strata that include a natural redox transition zone. These strata included the oxic coarse-grained Hanford formation, fine-grained oxic and anoxic Ringold Formation sediments, and the weathered basalt group. We detected 1233 and 120 unique bacterial and archaeal OTUs (operational taxonomic units at the 97% identity level) respectively. Microbial community structure and richness varied substantially across the different geological strata. Bacterial OTU richness (Chao1 estimator) was highest (> 700) in the upper Hanford formation, and declined to about 120 at the bottom of the Hanford formation. Just above the Ringold oxic-anoxic interface, richness was about 325 and declined to less than 50 in the deeper reduced zones. The deeper Ringold strata were characterized by a preponderance (c. 90%) of Proteobacteria. The bacterial community in the oxic sediments contained not only members of nine well-recognized phyla but also an unusually high proportion of three candidate divisions (GAL15, NC10 and SPAM). Additionally, 13 novel phylogenetic orders were identified within the Deltaproteobacteria, a clade rich in microbes that carry out redox transformations of metals that are important contaminants on the Hanford Site.