Proliferation of progenitor cells in the adult rat brain correlates with the presence of vimentin-expressing astrocytes
Proliferation of progenitor cells in the adult rat brain correlates with the presence of vimentin-expressing astrocytes
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作者：
Alonso, G
成熟的海马齿状回（DG）和侧脑室（SVZ）的脑室下区（SVZ）内存在祖细胞的增殖。本研究的目的是确定这些生发区内的细胞增殖率是否与特定神经胶质环境的发生相关。在已知改变祖细胞增殖速率的不同生理和实验条件下，向大鼠施用细胞增殖标记物溴脱氧尿苷（BrdU）。在这两个发芽区，BrdU标记的细胞核与免疫染色的神经元标记物聚唾液酸化神经细胞，粘附分子，但不是神经胶质细胞标记物胶质细胞酸性蛋白（GFAP）或波形蛋白（Vim）的细胞体。然而，在所有的大鼠检查，增殖（BrdU标记）细胞总是表现出密切的关系与不成熟的星形胶质细胞，表达GFAP和Vim。老年大鼠和皮质酮处理的成年大鼠DG中的细胞增殖显著减少，这与该区域中存在的星形胶质细胞的波形蛋白表达减少有关。相比之下，这两个治疗组的SVZ中细胞增殖和波形蛋白表达仅受到轻微影响。相反，无论是肾上腺切除术或手术损伤后，通过侧海马，在DG中观察到的细胞增殖的增加相关的GFAP和Vim双免疫染色结构在这些地区的数量增加的发生。总之，这些数据表明，在生发区存在的不成熟样星形胶质细胞可能提供了一个微环境，参与维持祖细胞的增殖。(C)2001 Wiley-Liss，Inc.
It is well established that proliferation of progenitor cells persists within the hippocampal dentate gyrus (DG) and the subventricular zone of the lateral ventricle (SVZ) in the adult brain. The aim of the present study was to determine whether the rate of cell proliferation within these germinative zones could be correlated to the occurrence of a particular glial environment. The cell proliferation marker bromodeoxyuridine (BrdU) was administrated to rats under different physiological and experimental conditions known to modify the rate of progenitor cell proliferation. Within both germinative zones, BrdU-labeled nuclei were associated with cell bodies immunostained for the neuronal marker polysialylated neural cell, adhesion molecule, but not for the glial markers glial fibrillary acidic protein (GFAP) or vimentin (VIM). In all the rats examined, however, proliferating (BrdU-labeled) cells always exhibited close relationships with immature-like astrocytes that expressed both GFAP and VIM. There was a dramatic decrease of cell proliferation in the DG from both the aged rats and the corticosterone-treated adult rats that was correlated with a decreased expression of vimentin by the astrocytes present in this region. In contrast, both cell proliferation and vimentin expression were only slightly affected in the SVZ from these two treatment groups. Conversely, after either adrenalectomy or a surgical lesion through the lateral hippocampus, the increase in cell proliferation observed in the DG was correlated to the occurrence of an increased number of GFAP and VIM double immunostained structures in these regions. All together, these data suggest that immature-like astrocytes present in the germinative zones may provide a microenvironment involved in sustaining the proliferation of progenitor cells. (C) 2001 Wiley-Liss, Inc.