A perspective of the dynamic structure of the nucleus explored at the single-molecule level.
A perspective of the dynamic structure of the nucleus explored at the single-molecule level.
复制标题
在单分子水平上探索的细胞核动态结构的透视图。
DOI：
10.1007/s10577-010-9156-5
复制
发表时间：
2011-01
影响因子：
2.6
通讯作者：
Grunwald, David
中科院分区：
文献类型：
--
作者：
Dange, Thomas;Joseph, Aviva;Grunwald, David
细胞生命可以被描述为一个高度复杂的相互作用分子网络的动态平衡。出于这个原因，“仅仅”知道细胞过程中参与者的身份已经不够了，还必须解决诸如在何处、何时以及持续多久等问题，以了解正在研究的机制。此外，集合测量可能不足以描述分子流动性、时空分辨率、动力学参数和地理绘图的各个步骤。重要的是要研究各个步骤到底发生在哪里，以增强我们对活细胞的理解。细胞核内，有太多高度复杂的多级过程，如复制、转录、剪接等，提供了一个复杂多样的景观。它的动力学研究，以一个新的水平的详细荧光相关光谱（FCS）。单分子跟踪，虽然在细胞生物学中仍处于起步阶段，但正在成为一种越来越有吸引力的方法来推断这种细胞器的关键元素。在这里，我们讨论了跟踪细胞核中的单个RNA和蛋白质的潜力。它们的动力学、定位和相互作用速率对我们理解细胞生命至关重要。为了证明这一点，我们提供了一个审查的艾滋病毒的生命周期，这是一个非常优雅的平衡核和细胞质的功能，并提供了一个机会，研究机制深入整合的结构内的细胞核。总之，我们的目标是提出一个具体的，动态的核细胞生命的基础上单分子和FCS数据，并提供了未来的前景。
Cellular life can be described as a dynamic equilibrium of a highly complex network of interacting molecules. For this reason, it is no longer sufficient to “only” know the identity of the participants in a cellular process, but questions such as where, when, and for how long also have to be addressed to understand the mechanism being investigated. Additionally, ensemble measurements may not sufficiently describe individual steps of molecular mobility, spatial-temporal resolution, kinetic parameters, and geographical mapping. It is vital to investigate where individual steps exactly occur to enhance our understanding of the living cell. The nucleus, home too many highly complex multi-order processes, such as replication, transcription, splicing, etc., provides a complicated, heterogeneous landscape. Its dynamics were studied to a new level of detail by fluorescence correlation spectroscopy (FCS). Single-molecule tracking, while still in its infancy in cell biology, is becoming a more and more attractive method to deduce key elements of this organelle. Here we discuss the potential of tracking single RNAs and proteins in the nucleus. Their dynamics, localization, and interaction rates will be vital to our understanding of cellular life. To demonstrate this, we provide a review of the HIV life cycle, which is an extremely elegant balance of nuclear and cytoplasmic functions and provides an opportunity to study mechanisms deeply integrated within the structure of the nucleus. In summary, we aim to present a specific, dynamic view of nuclear cellular life based on single molecule and FCS data and provide a prospective for the future.
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影响因子：
4.8
作者：
Cherepanov, P;Maertens, G;Debyser, Z
通讯作者：
Debyser, Z
影响因子：
64.5
作者：
Fornerod, M;Ohno, M;Mattaj, IW
通讯作者：
Mattaj, IW
影响因子：
56.9
作者：
Elf, Johan;Li, Gene-Wei;Xie, X. Sunney
通讯作者：
Xie, X. Sunney
影响因子：
14.9
作者：
Beitzel, B;Bushman, F
通讯作者：
Bushman, F
DOI：
10.1073/pnas.2233244100
发表时间：
2003-11-11
影响因子：
11.1
作者：
Bratu, DP;Cha, BJ;Tyagi, S
通讯作者：
Tyagi, S