Transplantation of beta cells from transgenic mice into nude athymic diabetic rats restores glucose regulation.
Transplantation of beta cells from transgenic mice into nude athymic diabetic rats restores glucose regulation.
复制标题
将转基因小鼠的β细胞移植到裸无胸腺糖尿病大鼠体内可以恢复葡萄糖调节。
DOI：
10.1016/0168-8227(91)90016-7
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发表时间：
1991
影响因子：
5.1
通讯作者：
Demetriou,AA
中科院分区：
文献类型：
--
作者：
Hicks,BA;Stein,R;Efrat,S;Grant,S;Hanahan,D;Demetriou,AA
我们已经证明，通过腹腔内移植来自小鼠胰腺β细胞系β TC-1的微载体附着的胰岛素产生β细胞，可以降低实验诱导的糖尿病裸无胸腺大鼠中升高的血浆葡萄糖水平。早在细胞移植后两天就观察到高血糖水平的降低，并且与血浆胰岛素水平的伴随增加相关。β TC-1细胞移植糖尿病大鼠的血浆葡萄糖水平与非糖尿病对照动物相似，并在整个39天实验期间保持血糖正常。β TC-1细胞移植糖尿病大鼠与对照糖尿病和非糖尿病大鼠相比，体重、摄食量、饮水量和尿量也接近正常。类似地，静脉内葡萄糖给药后，它们表现出改善的血糖清除率。这些结果表明，β TC-1细胞在移植到糖尿病大鼠受体后以类似于内源性胰腺β细胞的方式调节葡萄糖稳态。
We have shown that elevated plasmad-glucose levels in experimentally-induced diabetic nude athymic rats can be reduced by intraperitoneal transplantation of microcarrier-attached insulin producing β cells from the mouse pancreatic β cell line, β TC-1. The reduction in the level of hyperglycemia was observed as early as two days following cell transplantation and was associated with a concomitant increase in plasma insulin levels. β TC-1 cell transplanted diabetic rats had plasmad-glucose levels similar to those found in non-diabetic control animals and remained normoglycemic throughout the 39 day experimental period. The β TC-1 cell transplanted diabetic rats also had near normalization of body weight, food and water intake and of urine output when compared to control diabetic and non-diabetic rats. Similarly, they exhibited improved blood glucose clearance following intravenousd-glucose administration. These results suggest that β TC-1 cells regulated-glucose homeostasis following transplantation into diabetic rat recipients in a manner similar to that of endogenous pancreatic β cells.