Stem cell based tissue engineering for meniscus repair
Stem cell based tissue engineering for meniscus repair
复制标题
DOI：
10.1002/jbm.a.31480
复制
发表时间：
2008-05-01
影响因子：
4.9
通讯作者：
Yoo, Jung
中科院分区：
文献类型：
--
作者：
Angele, Peter;Johnstone, Brian;Yoo, Jung
半月板的缺陷极大地改变了膝关节的功能，并使关节易于发生退行性变化。本研究的目的是测试最近开发的细胞-支架组合用于修复兔内侧半月板的临界尺寸缺损。在18只新西兰白色兔中，对内侧半月板的中间部进行了双侧完全切除。将透明质酸/明胶复合支架植入6只兔的一侧膝关节缺损中，对侧缺损不作处理。将装载有自体骨髓间充质干细胞并在软骨形成培养基中培养14天的支架植入第二组12只兔子中。将空支架植入对侧膝关节中。在12周时收获半月板。未经治疗的缺损有一个温和的纤维愈合反应。用无细胞植入物治疗的缺陷也主要表现为纤维组织，而纤维软骨存在于一些支架中。用无细胞支架处理后修复组织的横截面宽度显著大于对照组（p < 0.05）。预培养的植入物与宿主组织整合，11例中有8例含有类软骨样纤维软骨，而11例对照中有2例含有类软骨样纤维软骨（p < 0.03）。预培养的种植体修复组织的平均横截面宽度大于对照组（p < 0.004）。本研究证明了用干细胞和基于支架的组织工程方法修复临界尺寸的尿道缺损。(C)2007 Wiley Periodicals，Inc.
Defects of the meniscus greatly alter knee function and predispose the joint to degenerative changes. The purpose of this study was to test a recently developed cell-scaffold combination for the repair of a critical-size defect of the rabbit medial meniscus. A bilateral, complete resection of the pars intermedia of the medial meniscus was performed in 18 New Zealand White rabbits. A hyaluronan/gelatin composite scaffold was implanted into the defect of one knee of 6 rabbits and the contralateral defect was left untreated. Scaffolds loaded with autologous marrow-derived mesenchymal stem cells and cultured in a chondrogenic medium for 14 days were implanted in a second series of 12 rabbits. Empty scaffolds were implanted in the contralateral knees. Meniscii were harvested at 12 weeks. Untreated defects had a muted fibrous healing response. Defects treated with cell-free implants showed also predominantly fibrous tissue whereas fibrocartilage was present in some scaffolds. The cross-sectional width of the repair tissue after treatment with cell-free scaffolds was significantly greater than controls (p < 0.05). Pre-cultured implants integrated with the host tissue and 8 of 11 contained meniscus-like fibrocartilage, compared with 2 of 11 controls (p < 0.03). The mean cross-sectional width of the pre-cultured implant repair tissue was greater than controls (p < 0.004). This study demonstrates the repair of a critical size meniscal defect with a stem cell and scaffold based tissue engineering approach. (C) 2007 Wiley Periodicals, Inc.