RhoA is associated with invasion and lymph node metastasis in upper urinary tract cancer
RhoA is associated with invasion and lymph node metastasis in upper urinary tract cancer
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DOI：
10.1046/j.1464-410x.2003.03063.x
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发表时间：
2003-02-01
影响因子：
4.5
通讯作者：
Yoshida, KI
中科院分区：
文献类型：
--
作者：
Kamai, T;Kawakami, S;Yoshida, KI
分析RhoA.PATIENTS和METHODS的mRNA和蛋白水平的RhoA的mRNA和蛋白水平的匹配组的肿瘤，非肿瘤和转移性淋巴结组织的手术标本进行了分析，在47例连续肾盂/输尿管癌患者，使用聚合酶链反应后，逆转录和蛋白质印迹法，以评估RhoA小GT酶（RhoA）在上尿路癌的作用。RhoA在肿瘤组织中的mRNA和蛋白水平与患者临床病理特征的关系也进行了评估，结果显示：RhoA在肿瘤组织和转移淋巴结组织中的mRNA和蛋白水平均高于非肿瘤组织（P均< 0.001）。RhoA mRNA和蛋白表达水平与肿瘤的低分化程度（P均< 0.05）和肌层浸润（P分别< 0.01和< 0.001）有关。低或高RhoA患者的Kaplan-Meier生存曲线显示，高mRNA和蛋白水平与较短的无病生存期（P < 0.01）和总生存期（P < 0.001）相关。考克斯比例风险模型多因素分析显示，RhoA蛋白高表达是无病生存期和总生存期的独立预后因素，仅次于临床分期（P均< 0.05）。
OBJECTIVETo assess the roles of RhoA small GTPase (RhoA) in upper urinary tract cancer by analysing the mRNA and protein levels of RhoA.PATIENTS AND METHODSThe mRNA and protein levels of RhoA in matched sets of tumour, non-tumour and metastatic lymph node tissues of surgical specimens were analysed in 47 consecutive patients with renal pelvic/ureteric cancer, using the polymerase chain reaction after reverse transcription and Western blotting. The relationship between mRNA and protein levels of RhoA in tumour tissues and the clinicopathological features of the patients was also assessed.RESULTSThe mRNA levels of RhoA and RhoA protein were greater in tumour and metastatic lymph node tissues than in non-tumour tissues (all P < 0.001). The expression levels of RhoA mRNA and protein levels in primary tumours was related to poorly differentiated grade (both P < 0.05) and muscle invasion (P < 0.01 and < 0.001, respectively). Kaplan-Meier plots of survival in patients with low or high RhoA showed that high mRNA and protein levels were associated with a shorter disease-free (P < 0.01) and overall survival (P < 0.001). Multivariate analysis using the Cox proportional hazards model showed that a high RhoA protein level was an independent prognostic factor, second to stage, in disease-free and overall survival (both P < 0.05).CONCLUSIONSThese findings suggest that RhoA is involved in the invasion and metastasis of upper urinary tract cancer, indicating that RhoA may be a useful prognostic factor in this disease.