GLUTAMATERGIC AND CHOLINERGIC INPUTS FROM THE PEDUNCULOPONTINE TEGMENTAL NUCLEUS TO DOPAMINE NEURONS IN THE SUBSTANTIA-NIGRA PARS COMPACTA
GLUTAMATERGIC AND CHOLINERGIC INPUTS FROM THE PEDUNCULOPONTINE TEGMENTAL NUCLEUS TO DOPAMINE NEURONS IN THE SUBSTANTIA-NIGRA PARS COMPACTA
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DOI：
10.1016/0168-0102(94)00869-h
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发表时间：
1995-02-01
影响因子：
2.9
通讯作者：
KITAI, ST
中科院分区：
文献类型：
--
作者：
FUTAMI, T;TAKAKUSAKI, K;KITAI, ST
在离体脑片上研究了电刺激脚桥被盖核（PPN）引起的黑质背侧部（SNc）多巴胺（DA）神经元的突触后反应。记录的神经元细胞内注射biocytin，也确定为DA神经元的免疫细胞化学技术。PPN刺激在DA能神经元上诱发单突触兴奋性突触后电位（EPSP），该电位由早期瞬时电位和慢电位组成。应用抗多巴胺能药物（1 mM犬尿烯酸和/或30 μ M 6-氰基-7-硝基喹喔啉-2，3-二酮（CNQX））在浴介质部分抑制EPSP，表明PPN输入SNc DA神经元是多巴胺能和非多巴胺能。抗胆碱能药物如阿托品、美加明和哌仑西平的应用抑制了抗多巴胺能耐药EPSP。这些数据表明收敛的pPN和胆碱能兴奋性输入SNc DA神经元和烟碱和毒蕈碱受体参与胆碱能传输。
Postsynaptic responses of dopamine (DA) neurons in the substantia nigra pars compacta (SNc) to stimulation of the pedunculopontine tegmental nuclei (PPN) were studied in in vitro slice preparations in the rat. The recorded neurons were intracellularly injected with biocytin and also identified as DA neurons by an immunocytochemical technique. PPN stimulation induced in DA neurons monosynaptic excitatory postsynaptic potentials (EPSPs) that consisted of early transient and slow components. An application of anti-glutamatergic agents (1 mM kynurenic acid and/or 30 mu M 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX)) in the bathing media partially suppressed the EPSPs, indicating that PPN inputs to SNc DA neurons are glutamatergic and non-glutamatergic. Anti-glutamatergic resistant EPSPs were suppressed by applications of anti-cholinergic agents such as atropine, mecamylamine, and pirenzepine. These data indicate a convergence of glutamatergic and cholinergic excitatory inputs from the PPN to SNc DA neurons and that both nicotinic and muscarinic receptors are involved in the cholinergic transmission.