Effect of mammalian cell culture medium on the gelation properties of Pluronic® F127
Effect of mammalian cell culture medium on the gelation properties of Pluronic® F127
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DOI：
10.1016/s0142-9612(02)00208-9
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发表时间：
2002-12-01
期刊：
影响因子：
14
通讯作者：
Bhatia, SR
中科院分区：
文献类型：
--
作者：
Matthew, JE;Nazario, YL;Bhatia, SR
我们研究了聚环氧乙烷-聚环氧丙烷-聚环氧乙烷共聚物Pluronic(R)F127在哺乳动物细胞培养介质中的凝胶化，用于组织工程和细胞封装。在最低基本培养液(MEM)和添加胎牛血清的MEM(MEM-FBS)中，与纯水相比，凝胶相边界移动到较低的温度和浓度。在MEM、MEM-FBS和纯水中凝胶形成的热力学相似，表明在这三种溶剂中凝胶的形成机理是相似的。溶胶-凝胶边界向较低浓度的转变对于使用Pluronics(R)的细胞封装方案的开发以及出于毒性考虑而必须将共聚物浓度降至最低的应用尤其重要。(C)2002爱思唯尔科学有限公司。保留所有权利。
We investigate the gelation of a poly(ethylene oxide)-polypropylene oxide)-polyethylene oxide) copolymer, Pluronic(R) F127, in mammalian cell culture medium for applications in tissue engineering and cell encapsulation. In both minimum essential medium (MEM) and MEM with added fetal bovine serum (MEM-FBS), the gel-phase boundary shifts to lower temperatures and concentrations as compared to pure water. The thermodynamics of gel formation are similar in MEM, MEM-FBS, and pure water, suggesting that the mechanism of gelation is similar in all three solvents. The shift of the sol-gel boundary to lower concentrations is particularly significant for development of cell encapsulation protocols using Pluronics(R) and applications where copolymer concentration must be minimized due to toxicity concerns. (C) 2002 Elsevier Science Ltd. All rights reserved.