Contraction Potency of Hypertrophic Scar‐derived Fibroblasts in a Connective Tissue Model: In Vitro Analysis of Wound Contraction
Contraction Potency of Hypertrophic Scar‐derived Fibroblasts in a Connective Tissue Model: In Vitro Analysis of Wound Contraction
复制标题
结缔组织模型中肥厚性疤痕衍生成纤维细胞的收缩能力：伤口收缩的体外分析
作者：
Chi;K. Hata;S. Torii;Mutsushi Matsuyama;M. Ueda
许多研究人员报道，胶原凝胶收缩反映了伤口收缩的机制。利用胶原凝胶格子结缔组织模型，我们分析了从增生性瘢痕、正常皮肤和正常口腔黏膜获得的成纤维细胞的收缩能力。然后通过免疫荧光显微镜和透射电子显微镜检查，试图分析中药收缩的机制。增生性瘢痕成纤维细胞与正常皮肤和正常口腔黏膜来源的CTM相比，具有最大的收缩能力和最短的滞后时间。可见，CTM的初始收缩程度与成纤维细胞向双极和拉长的形态变化密切相关。增生性瘢痕成纤维细胞突起延长较快，细胞内肌动蛋白细丝较正常成纤维细胞多。我们认为增生性瘢痕CTM是研究创面收缩和增生性瘢痕形成的一种有用的病理模型。
Many investigators have reported that collagen gel contraction reflects the mechanism of wound contraction. By using a connective tissue model (CTM) of collagen gel lattice, we analyzed the contraction potency of fibroblasts that had been obtained from hypertrophic scar, normal skin, and normal oral mucosa. We then tried to analyze the mechanism of CTM contraction by immunofluorescent microscopic and transmission electron microscopic examinations. Hypertrophic scar-derived fibroblasts in CTM possessed the greatest contraction potency and the shortest lag time when compared with those of normal skin and normal oral mucosa-derived CTMs. It became clear that the initial contractive degree of CTM was closely related to morphological changes of fibroblast cells into bipolar and elongated shapes. Hypertrophic scar-derived fibroblasts elongated their processes faster, and their intracellular actin filaments were more numerous than those of normal fibroblasts. We believe that the hypertrophic scar-derived CTM is a useful pathological model for the research of wound contraction and hypertrophic scar formation.