Analysis of MT-45, a Novel Synthetic Opioid, in Human Whole Blood by LC-MS-MS and Its Identification in a Drug-Related Death
Analysis of MT-45, a Novel Synthetic Opioid, in Human Whole Blood by LC-MS-MS and Its Identification in a Drug-Related Death
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DOI：
10.1093/jat/bkw012
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发表时间：
2016-05-01
影响因子：
2.5
通讯作者：
Logan, Barry K.
中科院分区：
文献类型：
--
作者：
Papsun, Donna;Krywanczyk, Alison;Logan, Barry K.
MT-45（1-环己基-4-（1,2-二苯基乙基）哌嗪）只是二十一世纪进入休闲毒品市场的众多新型精神活性物质（NPS）之一；然而，在目前由合成大麻素和设计兴奋剂主导的市场中，它是首批获得一定程度受欢迎的设计阿片类药物之一。描述了一起涉及 MT-45 和依替唑仑的死亡事件。开发并验证了使用液相色谱-串联质谱法定量全血中 MT-45 的方法。确定线性范围为1.0-100 ng/mL，检测限为1.0 ng/mL，方法满足可接受的线性、精密度和准确度要求。对样品进行稀释和标准添加分析后，确定死者血液中 MT-45 的浓度为 520 ng/mL，与药物相关死亡中报告的其他 MT-45 浓度一致。依替唑仑的浓度为 35 ng/mL。这个案例说明了在不明原因的明显与药物相关的死亡中考虑非传统药物的重要性。
MT-45 (1-cyclohexyl-4-(1,2-diphenylethyl)piperazine) is just one of the many novel psychoactive substances (NPS) to have reached the recreational drug market in the twenty-first century; it is however, one of the first designer opioids to achieve some degree of popularity, in a market currently dominated by synthetic cannabinoids and designer stimulants. A single fatality involving MT-45 and etizolam is described. A method for the quantitation of MT-45 in whole blood using liquid chromatography-tandem mass spectrometry was developed and validated. The linear range was determined to be 1.0-100 ng/mL with a detection limit of 1.0 ng/mL, and the method met the requirements for acceptable linearity, precision and accuracy. After analyzing the sample on dilution and by standard addition, the concentration of MT-45 in the decedent's blood was determined to be 520 ng/mL, consistent with other concentrations of MT-45 reported in drug-related fatalities. Etizolam was present at a concentration of 35 ng/mL. This case illustrates the importance of considering non-traditional drugs in unexplained apparent drug-related deaths.