Binding of taxol to plastic and glass containers and protein under in vitro conditions.
Binding of taxol to plastic and glass containers and protein under in vitro conditions.
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在体外条件下紫杉醇与塑料和玻璃容器以及蛋白质的结合。
DOI：
10.1021/js950286j
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Au,JL
中科院分区：
文献类型：
--
作者：
Song,D;Hsu,LF;Au,JL
本文研究了紫杉醇在水溶液和组织培养液中的稳定性和蛋白结合。在1%甲醇水溶液中储存19 h后，紫杉醇的浓度在1.5 mL玻璃小瓶中下降至约40%，在1.5 mL聚丙烯管中下降至67%，在1.5 mL硅化聚丙烯管中下降至55%。在0.18和1.8μ g/mL的两个初始浓度下，下降没有差异。浓度下降是双相的，初始阶段显示半衰期为1-2分钟，第二阶段显示半衰期为30-350分钟。甲醇洗涤回收0%，16%和31%的紫杉醇从玻璃小瓶和未硅化和硅化聚丙烯管，分别。在不含胎牛血清的培养基中，储存在聚苯乙烯组织培养板中的紫杉醇浓度在24 h后下降至73%，而在存在9%胎牛血清（FBS）的情况下没有观察到浓度下降。在含FBS的培养基中，紫杉醇的蛋白结合是饱和的，从0.5 μg/mL的79%到15 μg/mL的20%。这些数据表明：（a）紫杉醇在塑料和玻璃表面上的快速和非特异性吸附;（B）FBS对培养基中紫杉醇的稳定性;（c）培养基中紫杉醇的蛋白结合是饱和的
This study examined the stability and protein binding of taxol in aqueous solution and tissue culture medium. After storage for 19 h in 1% methanolic aqueous solution, the concentration of taxol declined to about 40% in 1.5 mL glass vials, 67% in 1.5 mL polypropylene tubes, and 55% in 1.5 mL siliconized polypropylene tubes. There was no difference in the decline at two initial concentrations of 0.18 and 1.8μglmL. The concentration declines were biphasic; the initial phase showed a half-life of 1-2 min and the second phase showed a half-life of 30-350 min. A methanol wash recovered 0%, 16%, and 31% of taxol from glass vials and unsiliconized and siliconized polypropylene tubes, respectively. In culture medium without fetal bovine serum, the concentration of taxol stored in polystyrene tissue culture plates declined to 73% after 24 h, whereas no concentration decline was observed in the presence of 9% fetal bovine serum (FBS). Protein binding of taxol in FBS-containing culture medium was saturable, varying from 79% at 0.5 μg/mL to 20% at 15 μg/mL These data indicated (a) rapid and nonspecific adsorption of taxol to plastic and glass surfaces, (b) stabilization of taxol in culture medium by FBS, and (c) saturable protein binding of taxol in culture medium
使用自动柱切换对生物体液和组织中的紫杉醇进行等度高效液相色谱分析。
DOI：
10.1016/0378-4347(94)00456-f
发表时间：
1995
期刊：
Journal of chromatography. B, Biomedical applications
影响因子：
--
作者：
Song,D;Au,JL
通讯作者：
Au,JL