Antibacterial and anti-inflammatory activities of extract and fractions from Pyrrosia petiolosa (Christ et Bar.) Ching
Antibacterial and anti-inflammatory activities of extract and fractions from Pyrrosia petiolosa (Christ et Bar.) Ching
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DOI：
10.1016/j.jep.2014.07.029
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发表时间：
2014-09-11
影响因子：
5.4
通讯作者：
Zhang, Hongmeng
中科院分区：
文献类型：
--
作者：
Cheng, Dandan;Zhang, Yingying;Zhang, Hongmeng
民族药理学相关性：小叶红是一种常用的中药，用于治疗急性肾盂肾炎、慢性支气管炎和支气管哮喘。本研究旨在评价不同极性溶剂制备的小叶红乙醇提取物及其衍生组分的抑菌活性，并进行抗炎筛选。材料与方法：采用乙醇、石油醚、乙酸乙酯、正丁醇、水萃取法提取小叶红的各组分。对乙醇提取物、石油醚馏分、乙酸乙酯馏分、正丁醇馏分和水馏分进行了定性植物化学筛选。采用琼脂扩散法、最低抑菌浓度（MIC）和最低杀菌浓度（MBC）评价乙醇提取物及其组分的抑菌活性。采用MTT法测定乙醇提取物及其组分的体外细胞毒性。采用二甲苯致小鼠耳肿胀法分析其抗炎活性。结果：经植物化学成分筛选，黄酮类、萜类、甾体、皂苷类、酚类、还原糖等化合物均存在。抑菌结果表明，石油醚部分和正丁醇部分对所有微生物均有抑制作用，最大抑制范围为15.25 +/- 0.35 mm。除铜绿假单胞菌ATCC 27853外，乙酸乙酯部位也表现出良好的抑菌活性，而提取物和水相部位对13种微生物中的8种（61.5%）具有抑制作用。乙醇提取物和馏分的MIC值在1.25 ~ 10.00 mg/mL之间，大部分MBC值等于或两倍于相应的MIC值。体外细胞毒性实验表明，乙醇提取物及其组分对肺癌细胞株A549和小鼠脾细胞具有无毒或低毒活性。在抗炎实验中，5.0和10.0 mg/kg乙醇提取物对二甲苯致小鼠耳肿胀具有显著的抗炎活性，最大抑制率高达67%。结论：小叶红是一种新型抗菌抗炎药。2014爱思唯尔爱尔兰有限公司版权所有。
Ethnopharmacological relevance: Pyrrosia petiolosa is commonly used as a traditional Chinese medicine for treatment of acute pyelonephritis, chronic bronchitis and bronchial asthma. This study aims to evaluate the antibacterial activity of the ethanol extract and its derived fractions of Pyrrosia petiolosa obtained with solvents of different polarities and to perform the anti-inflammatory screening.Materials and methods: The powdered aerial parts of Pyrrosia petiolosa were used to extract various fractions with ethanol, petroleum ether, ethyl acetate, N-butanol and aqueous. Qualitative phytochemical screening was performed on the ethanol extract, petroleum ether fraction, ethyl acetate fraction, N-butanol fraction and aqueous fraction. The agar diffusion method, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were employed to evaluate antibacterial activity of the ethanol extract and fractions. The in vitro cytotoxicity of ethanol extract and fractions was determined using MTT assay. The anti-inflammatory activity was analyzed using the mouse ear swelling induced by xylene.Results: The phytochemical screening revealed the presence of anthraquinones, flavonoids, terpenoids, steroids, saponins, phenols and reducing sugars in the extract and fractions. Antibacterial results showed that petroleum ether fraction and N-butanol fraction inhibited all the tested microorganisms with the maximum inhibition zone of 15.25 +/- 0.35 mm. Ethyl acetate fraction also exhibited good antibacterial activity except Pseudomonas aeruginosa ATCC 27853, while extract and aqueous fraction inhibited 8 out of 13 (61.5%) of the tested microorganisms. The MIC values of ethanol extract and fractions ranged from 1.25 to 10.00 mg/mL and most of the MBC values were equal or twice as high as the corresponding MIC values. The in vitro cytotoxicity showed the ethanol extract and fractions exhibited non-toxic or low toxic activity against lung cancer cell lines A549 and mouse spleen cells. In anti-inflammatory experiment, ethanol extract at 5.0 and 10.0 mg/kg exhibited significant anti-inflammatory activity against the mouse ear swelling induced by xylene and the maximum inhibition rate reached as high as 67%.Conclusions: Pyrrosia petiolosa could be a potential candidate for future development of a novel antibacterial and anti-inflammatory agent. (C) 2014 Elsevier Ireland Ltd. All rights reserved.