Surface alterations of erythrocytes with cell age: rat red cell is not a model for human red cell.
Surface alterations of erythrocytes with cell age: rat red cell is not a model for human red cell.
复制标题
红细胞表面随细胞年龄的变化：大鼠红细胞不是人类红细胞的模型。
DOI：
10.1016/s0006-291x(80)80141-0
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发表时间：
1980
影响因子：
3.1
通讯作者：
Seaman,GV
中科院分区：
文献类型：
--
作者：
Walter,H;Krob,EJ;Tamblyn,CH;Seaman,GV
人和大鼠的年轻和年老的红细胞的电荷相关的表面特性进行了检查，通过在葡聚糖-聚（乙二醇）相系统的水相之间具有静电电位差和电泳的分区。发现（a）大鼠红细胞经历表面电荷相关性质的变化，这是细胞年龄的函数，可通过分配检测到，而人红细胞不经历这种变化;（B）逆流分布后的分析性粒子电泳证实了大鼠红细胞中的变化和人红细胞中的不存在;和（c）在没有预先细胞分离的情况下，粒子电泳不能检测大鼠红细胞中与年龄相关的电荷相关改变。
The charge-related surface properties of human and rat young and old erythrocytes were examined by partitioning in an aqueous dextran-poly(ethylene glycol) phase system having an electrostatic potential difference between the phases and by electrophoresis. It was found (a) that while rat red cells undergo changes in surface charge-related properties as a function of cell age which are detectable by partitioning, human erythrocytes do not; (b) that analytical particle electrophoresis after countercurrent distribution confirms both the alteration in rat and its absence in human erythrocytes; and (c) that particle electrophoresis cannot detect the age-related charge-associated alteration in rat red cells without prior cell partitioning.