Glucose Uptake in Prochlorococcus: Diversity of Kinetics and Effects on the Metabolism.
Glucose Uptake in Prochlorococcus: Diversity of Kinetics and Effects on the Metabolism.
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DOI：
10.3389/fmicb.2017.00327
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发表时间：
2017
影响因子：
5.2
通讯作者：
García-Fernández JM
中科院分区：
文献类型：
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作者：
Muñoz-Marín MD;Gómez-Baena G;Díez J;Beynon RJ;González-Ballester D;Zubkov MV;García-Fernández JM
我们之前已经证明原绿球藻属。 SS120 菌株通过使用 Pro1404 基因编码的多相转运蛋白吸收葡萄糖。在这里，我们研究了来自不同生态型的多种原绿球菌菌株的葡萄糖摄取动力学，观察了不同的 Ks 常数值 (15–126.60 nM) 和摄取率 (0.48–6.36 pmol min-1 mg prot-1)。除 TAK9803-2 外，所有研究菌株均观察到多相动力学。第 21 次大西洋经线断面巡航期间的 Pro1404 基因表达研究显示与海洋中的葡萄糖浓度呈正相关。这表明 Pro1404 转运蛋白在原绿球藻的进化过程中经历了多样化，这一过程可能是由其所栖息的不同生态位的葡萄糖可用性驱动的。葡萄糖摄取机制似乎是主要转运蛋白。添加葡萄糖在原绿球藻 SS120 中引起可检测到的转录组和蛋白质组变化，但光合效率不受影响。我们的研究表明，原绿球藻主动吸收葡萄糖，但其吸收并没有显着改变这种继续进行光合作用的蓝藻的营养方式。因此，原绿球藻似乎仍然是一种基本的光养生物，能够利用环境中的葡萄糖作为额外的碳和能量资源。
We have previously shown that Prochlorococcus sp. SS120 strain takes up glucose by using a multiphasic transporter encoded by the Pro1404 gene. Here, we studied the glucose uptake kinetics in multiple Prochlorococcus strains from different ecotypes, observing diverse values for the Ks constants (15–126.60 nM) and the uptake rates (0.48–6.36 pmol min-1 mg prot-1). Multiphasic kinetics was observed in all studied strains, except for TAK9803-2. Pro1404 gene expression studies during the 21st Atlantic Meridional Transect cruise showed positive correlation with glucose concentrations in the ocean. This suggests that the Pro1404 transporter has been subjected to diversification along the Prochlorococcus evolution, in a process probably driven by the glucose availabilities at the different niches it inhabits. The glucose uptake mechanism seems to be a primary transporter. Glucose addition induced detectable transcriptomic and proteomic changes in Prochlorococcus SS120, but photosynthetic efficiency was unaffected. Our studies indicate that glucose is actively taken up by Prochlorococcus, but its uptake does not significantly alter the trophic ways of this cyanobacterium, which continues performing photosynthesis. Therefore Prochlorococcus seems to remain acting as a fundamentally phototrophic organism, capable of using glucose as an extra resource of carbon and energy when available in the environment.