Effect of methionine loading on 5-methyltetrahydrofolate, S-adenosylmethionine and S-adenosylhomocysteine in plasma of healthy
Effect of methionine loading on 5-methyltetrahydrofolate, S-adenosylmethionine and S-adenosylhomocysteine in plasma of healthy
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DOI：
10.1042/cs0910079
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发表时间：
1996-07-01
期刊：
影响因子：
6
通讯作者：
Fowler, B
中科院分区：
文献类型：
--
作者：
Loehrer, FMT;Haefei, WE;Fowler, B
1.血浆同型半胱氨酸浓度升高，无论是在空腹状态还是蛋氨酸负荷后，都是人类血管疾病的独立危险因素，蛋氨酸负荷已被用于研究蛋氨酸代谢受损，尤其是转硫途径，但大多数研究集中在同型半胱氨酸的变化.我们研究了12名健康受试者在24 h内蛋氨酸过量对血浆总同型半胱氨酸、5-甲基四氢叶酸（叶酸在同型半胱氨酸再甲基化为蛋氨酸过程中的活性形式）、S-腺苷蛋氨酸（蛋氨酸的第一代谢产物）和S-腺苷同型半胱氨酸（S-腺苷蛋氨酸的脱甲基产物）的影响.以及高半胱氨酸的预期增加（从8.0+/-1.3至32.6+/-10.3 μ mol/l，平均值+/-SD P
1. Elevated plasma homocysteine concentration, either in the fasting state or after methionine loading, is an independent risk factor for vascular disease in man. Methionine loading has been used to investigate impaired methionine metabolism, especially of the trans-sulphuration pathway, but most studies have focused on changes in homocysteine.2. We investigated the effect of methionine excess on total plasma homocysteine, 5-methyltetrahydrofolate (which is the active form of folate in the remethylation of homocysteine to methionine), S-adenosylmethionine (the first metabolite of methionine) and S-adenosylhomocysteine (the demethylated product of S-adenosylmethionine) over 24 h in 12 healthy subjects.3. As well as the expected increase in homocysteine (from 8.0+/-1.3 to 32.6+/-10.3 mu mol/l, mean+/-SD P