Establishment and characterization of a new human mesothelioma cell line (T‐85) from malignant peritoneal mesothelioma with remarkable thrombocytosis
Establishment and characterization of a new human mesothelioma cell line (T‐85) from malignant peritoneal mesothelioma with remarkable thrombocytosis
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具有显着血小板增多症的恶性腹膜间皮瘤的新人间皮瘤细胞系（T-85）的建立和表征
DOI：
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发表时间：
1995
期刊：
影响因子：
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通讯作者：
R. Machinami
中科院分区：
文献类型：
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作者：
T. Tange;Y. Hasegawa;T. Oka;S. Sunaga;M. Higashihara;K. Matsuo;H. Miyazaki;A. Shimosaka;A. Okano;K. Todokoro;T. Ishikawa;R. Machinami
间皮瘤细胞系，称为T-85，是从患有恶性腹膜间皮瘤和显著血小板增多症（1.4 × 106/mm 3）的患者中建立的。电子显微镜下，两种类型的间皮瘤细胞已被表征;细胞质中具有致密核心颗粒的主要类型的细胞和具有均匀致密颗粒的次要类型。免疫学上，细胞显示白细胞介素-6（IL-6）、细胞角蛋白、IV型胶原蛋白、波形蛋白、层粘连蛋白、纤连蛋白和因子VIII相关抗原染色。ELISA定量显示T-85细胞培养上清液中IL-6浓度较高。T-85细胞的RT-聚合酶链反应在628和251碱基对处显示两条cDNA阳性条带，表明IL-6和IL-6受体mRNA的组成性表达。此外，T-85细胞培养上清液中显著的促血小板过程形成活性表明存在主要由IL-6而非c-MpI配体或促红细胞生成素引起的血小板生成活性。然而，用抗IL-6抗体处理的上清液中仍保留15%的PPF活性，这一事实表明存在另一种血小板生成物质。T-85是迄今为止第一个间皮瘤细胞系来自一个明显的血小板增多症的情况下。
A mesothelioma cell line, termed T‐85, was established from a patient with malignant peritoneal mesothelioma and remarkable thrombocytosis (1.4 × 106/mm3). Electron microscopically, two types of mesothelioma cells have been characterized; the major type of cells with dense‐cored granules in the cytoplasm and the minor one with evenly dense granules. Immunologically, the cells showed staining for inter‐leukin‐6 (IL‐6), cytokeratin, collagen type IV, vimentin, laminin, fibronectin and Factor VIII‐related antigen. Quanti‐tation by ELISA revealed a high concentration of IL‐6 in T‐85 cell culture supernatants. RT‐polymerase chain reaction of T‐85 cells showed two positive bands of cDNA at 628 and 251 base pairs indicating the constitutive expression of IL‐6 and IL‐6 receptor mRNA. Moreover, prominent pro‐platelet process formation activity in T‐85 cell culture supernatants indicated the presence of a thrombopoietic activity due mainly to IL‐6 but not the c‐MpI ligand or erythropoietin. However, the fact that 15% of PPF activity remained in the supernatants treated with anti‐IL‐6 antibody indicated the presence of another thrombopoietic substance. T‐85 is so far the first mesothelioma cell line derived from a case with remarkable thrombocytosis.
影响因子：
20.3
作者：
G. Demetri;Beatrice W. Zenzie;J. Rheinwald;J. Griffin
通讯作者：
G. Demetri;Beatrice W. Zenzie;J. Rheinwald;J. Griffin
人类恶性间皮瘤细胞系（H-MESO-1）的表征：双相实体和腹水肿瘤模型。
影响因子：
11.2
作者：
Reale,FR;Griffin,TW;Compton,JM;Graham,S;Townes,PL;Bogden,A
通讯作者：
Bogden,A