A nodule-specific protein secretory pathway required for nitrogen-fixing symbiosis.
A nodule-specific protein secretory pathway required for nitrogen-fixing symbiosis.
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DOI：
10.1126/science.1184096
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发表时间：
2010-02-26
期刊：
影响因子：
--
通讯作者：
Long S
中科院分区：
文献类型：
--
作者：
Wang D;Griffitts J;Starker C;Fedorova E;Limpens E;Ivanov S;Bisseling T;Long S
苜蓿中华根瘤菌与其豆科寄主植物蒺藜苜蓿之间的固氮共生发生在一种称为根瘤的特化根器官中。释放到植物细胞中的细菌被称为共生体的独特植物膜隔室包围。我们发现，在共生缺陷型的M.蒺藜、类杆菌和共生体发育受阻。我们确定了DNF1基因编码的信号肽酶复合物的一个亚基，是高度表达的结节。通过分析全基因组表达分析的数据，我们提出正确的共生体发育在M。蒺藜需要通过协调上调以DNF1为例的结节特异性途径来有序分泌蛋白质组分。
The nitrogen-fixing symbiosis between Sinorhizobium meliloti and its leguminous host plant Medicago truncatula occurs in a specialized root organ called the nodule. Bacteria that are released into plant cells are surrounded by a unique plant membrane compartment termed a symbiosome. We found that in the symbiosis-defective dnf1 mutant of M. truncatula, bacteroid and symbiosome development are blocked. We identified the DNF1 gene as encoding a subunit of a signal peptidase complex that is highly expressed in nodules. By analyzing data from whole-genome expression analysis, we propose that correct symbiosome development in M. truncatula requires the orderly secretion of protein constituents through coordinated up-regulation of a nodule-specific pathway exemplified by DNF1.