Three-step purification of glucocorticoid receptors from rat liver.
Three-step purification of glucocorticoid receptors from rat liver.
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从大鼠肝脏中三步纯化糖皮质激素受体。
DOI：
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发表时间：
1980
期刊：
影响因子：
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通讯作者：
B. Manz
中科院分区：
文献类型：
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作者：
M. Govindan;B. Manz
The 90 000-Mr glucocorticoid receptor was purified to homogeneity according to sodium dodecylsulfate/polyacrylamide gel electrophoresis. An affinity column containing either dexamethasone-17 beta-carboxylic acid or dexamethasone-21-methanesulfonate bound to the matrix with the help of a disulfide bond is used in this study. Using this affinity matrix, in a single step, 8700-fold purification of glucocorticoid receptor from rat liver cytosol could be achieved. Following the method of activation and DNA-cellulose chromatography the 90 000-Mr receptor subunit was purified to homogeneity.
