ELECTRON-MICROSCOPY OF NON-NEURONAL CELLULAR CHANGES ACCOMPANYING NEURAL DEGENERATION IN THALAMIC NUCLEI OF RABBIT .2. REACTIVE ELEMENTS WITHIN NEUROPIL
ELECTRON-MICROSCOPY OF NON-NEURONAL CELLULAR CHANGES ACCOMPANYING NEURAL DEGENERATION IN THALAMIC NUCLEI OF RABBIT .2. REACTIVE ELEMENTS WITHIN NEUROPIL
复制标题
DOI：
10.1002/cne.901480303
复制
发表时间：
1973-01-01
影响因子：
2.5
通讯作者：
KRUGER, L
中科院分区：
文献类型：
--
作者：
MATTHEWS, MA;KRUGER, L
Surgical destruction of the cortical projections of thalamic neurons results in rapid and profound atrophy of these neurons accompanied by a variety of non‐neuronal reactive changes. During the first post‐operative week, non‐neuronal elements, distinct from astrocytes and normal oligodendrocytes and tentatively designated as “M” cells, become evident in the degenerating thalamic nuclei. Some “M” cells are characterized by heterochromatin nuclei and numerous free ribosomes, and resemble the agranular leukocytes accumulating simultaneously within the perivascular space of small vessels coursing through these regions (Matthews and Kruger, '73). Examples of such cells were sometimes seen bridging the external basal lamina. Other “M” cells display many profiles of granular endoplasmic reticulum and fewer free ribosomes but cannot be precisely classified as to nature and possible source. From the second through fourteenth week, “M” cells increase in prominence and accumulate electronlucent lipid vacuoles and phosphotungstic acid (PTA) positive dense bodies, presumably lysosomes, until these enlarged lipid‐laden cells constitute the predominant non‐neuronal entities in the zone of degeneration, in addition to hypertrophied astrocytes. Thirty‐four weeks post‐operatively “M” cells have decreased in size and apparent numbers, and dense bodies display a lessened affinity for PTA in addition to morphologic patterns suggestive of terminal lysosomes. Normal light and dark oligodendrocytes are encountered within the zone of degeneration throughout the entire survival period.It is suggested that a significant proportion of “M” cells are of mesodermal (hematogenous; perivascular) origin although “M” cells display sufficient diversity of morphology to warrant consideration of additional sources for the reactive vacuolated forms. The experimental observations are discussed in relation to past and present concepts concerning the nature, origin and pathologic transformation of elements classified as microglia.
