DCNA CLONING AND SEQUENCE OF MAL, A HYDROPHOBIC PROTEIN ASSOCIATED WITH HUMAN T-CELL DIFFERENTIATION
DCNA CLONING AND SEQUENCE OF MAL, A HYDROPHOBIC PROTEIN ASSOCIATED WITH HUMAN T-CELL DIFFERENTIATION
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DOI：
10.1073/pnas.84.7.1997
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发表时间：
1987-04-01
影响因子：
11.1
通讯作者：
WEISSMAN, SM
中科院分区：
文献类型：
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作者：
ALONSO, MA;WEISSMAN, SM
我们已经分离出一种在T细胞分化的中期和晚期表达的人类cDNA。该cDNA编码一种高度疏水的蛋白质，称为MAL，它缺乏疏水的引导肽序列，并包含四个由短亲水片段分隔的潜在跨膜结构域。MAL蛋白的预测构象类似于在质膜中形成孔道或通道的整合蛋白的结构，并且据信其作为水溶性分子和离子穿过脂质双层的转运体发挥作用。在一组同时表达T细胞受体和T11抗原的T细胞系中存在MAL mRNA，这表明MAL可能参与通过T11或T细胞受体途径激活的T细胞中的膜信号传导。
We have isolated a human cDNA that is expressed in the intermediate and late stages of T-cell differentiation. The cDNA encodes a highly hydrophobic protein, termed MAL, that lacks a hydrophobic leader peptide sequence and contains four potential transmembrane domains separated by short hydrophilic segments. The predicted configuration of the MAL protein resembles the structure of integral proteins that form pores or channels in the plasma membrane and that are believed to act as transporters of water-soluble molecules and ions across the lipid bilayer. The presence of MAL mRNA in a panel of T-cell lines that express both the T-cell receptor and the T11 antigen suggests that MAL may be involved in membrane signaling in T cells activated via either T11 or T-cell receptor pathways.