Kinetoplastid mitochondria contain functional tRNAs which are encoded in nuclear DNA and also contain small minicircle and maxicircle transcripts of unknown function.
Kinetoplastid mitochondria contain functional tRNAs which are encoded in nuclear DNA and also contain small minicircle and maxicircle transcripts of unknown function.
复制标题
动质体线粒体含有在核 DNA 中编码的功能性 tRNA，还含有功能未知的小小环和大环转录物。
DOI：
10.1093/nar/17.14.5427
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发表时间：
1989
影响因子：
14.9
通讯作者：
Simpson,L
中科院分区：
文献类型：
--
作者：
Simpson,AM;Suyama,Y;Dewes,H;Campbell,DA;Simpson,L
The mitochondrion ofLeishmania tarentolaecontains approximately 35—40 tRNAs, many of which comigrate with cytoplasmic tRNAs. Both mitochondrial (KtRNA) and cytoplasmic (CtRNA) tRNAs are functional, as they could be acylated either by mitochondrial or cytoplasmic synthetase extracts. There are two methionyl tRNA species in the cytoplasmic and mitochondrial fractions, one of which is unique to each fraction, indicating that the KtRNA fraction is free of CtRNA contamination. Leucyl and glycyl tRNAs were identified by hybridization with a genomic clone from Trypanosoma brucei. KtRNA hybridizes with nuclear chromosomes, but not with minicircle or maxicircle DNA. KtRNA isolated by DEAE chromatography or agarose gel electrophoresis contains additional small RNAs which hybridize with both minicircle and maxicircle DNA. These transcripts do not migrate like tRNAs in acrylamide gels and their function is unknown. We suggest that most if not all mitochondrial tRNAs in L tarentolae are nuclear-encoded and imported into the mitochondrion.
