Beneficial effect of tripterine on systemic lupus erythematosus induced by active chromatin in BALB/c mice
Beneficial effect of tripterine on systemic lupus erythematosus induced by active chromatin in BALB/c mice
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DOI：
10.1016/j.ejphar.2005.02.030
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发表时间：
2005-04-11
影响因子：
5
通讯作者：
Li, D
中科院分区：
文献类型：
--
作者：
Li, H;Zhang, YY;Li, D
本研究的目的是确定从雷公藤中分离的雷公藤红素是否对BALB/c小鼠活性染色诱导的实验性系统性红斑狼疮有有益作用。在第0天用从豆豆蛋白a激活的同质脾淋巴细胞中分离的活性染色质免疫BALB/c小鼠。从第35天至第50天，给BALB/c小鼠灌胃雷公藤红素6或12 mg kg（-1）天（-1），或强的松5 mg kg（-1）天（-1）。用雷公藤红素12 mg kg（-1）天（-1）治疗15天，可保护肾脏免受肾小球损伤，同时降低血清自身抗体和总免疫球蛋白G (IgG)，并改善由豆豆蛋白a和脂多糖刺激的脾细胞增殖。与对照组相比，这种作用与白细胞介素-10的产生和血清一氧化氮（NO）水平的降低有关，但与干扰素- γ无关。雷公藤红素6 kg(-1) d（-1）对肾小球损伤无显著保护作用。它抑制自身抗体和白细胞介素- 10的产生，但对脾细胞增殖、血清no水平和干扰素- γ的产生没有影响。这些结果提示雷公藤红素对BALB/c小鼠活性染色质诱导的系统性红斑狼疮有有益作用。(c) 2005 Elsevier B.V.版权所有
The aim of this study was to determine whether tripterine, isolated from Tripterygium wilfordii Hoog f. in China, had beneficial effects on experimental systemic lupus erythematosus induced by active chromatin in BALB/c mice. BALB/c mice were immunized with active chromatin isolated from concanavalin A-activated syngenctic spleno-lymphocytes on day 0. Tripterine 6 or 12 mg kg(-1) day(-1), or prednisone 5 mg kg(-1) day(-1) was given to BALB/c mice intragastrically from day 3 5 to day 50. Treatment with tripterine 12 mg kg(-1) day(-1) for 15 days protected renal from glomerular injury with a concomitant reduction of serum autoantibodies and total immunoglobulin G (IgG) also with a improvement of splenocyte proliferation stimulated with concanavalin A and lipopolysaccharide. The effects were associated with reduced interleukin-10 production and serum nitric oxide (NO) level but not interferon-gamma compared with vehicle-treated control group. Tripterine 6 ing kg(-1) day(-1) had no significant protective effect against glomerular injury. It inhibited autoantibodies and interleukin- 10 production but had no effect on splenocyte proliferation, serum NO level, and interferon-gamma production. These findings suggested that tripterine had a beneficial effect on systemic lupus erythematosus induced by active chromatin in BALB/c mice. (c) 2005 Elsevier B.V. All rights reserved.