Biocompatible, Flexible, and Oxygen-Permeable Silicone-Hydrogel Material for Stereolithographic Printing of Microfluidic Lab-On-A-Chip and Cell-Culture Devices
Biocompatible, Flexible, and Oxygen-Permeable Silicone-Hydrogel Material for Stereolithographic Printing of Microfluidic Lab-On-A-Chip and Cell-Culture Devices
复制标题
用于微流控芯片实验室和细胞培养装置的立体光刻印刷的生物相容性、柔性和透氧硅水凝胶材料
DOI：
10.1021/acsapm.0c01071
复制
发表时间：
2021-01-08
影响因子：
5
通讯作者：
Wolfrum，Bernhard
中科院分区：
文献类型：
--
作者：
Zips，Sabine;Hiendlmeier，Lukas;Wolfrum，Bernhard
我们提出了一种光固化的、生物相容性的、柔性的硅水凝胶混合材料，用于生物医学设备的立体光刻（SLA）印刷。这种硅水凝胶聚合物类似于用于隐形眼镜的混合物。它具有弹性和可拉伸性，杨氏模量为78 MPa，最大断裂伸长率为51%，在水中膨胀程度低（<4% v/v），并且可以通过表面改性方法轻松粘合到平板玻璃基板上。通过WST-8细胞活力测定评估材料的玻璃细胞毒性，使用五种不同的细胞系：HT1080、L929和Hs27成纤维细胞、心肌细胞样HL-1细胞和神经元表型PC-12细胞。因此，将硅水凝胶聚合物与用于细胞培养应用和聚二甲基硅氧烷（PDMS）的其他几种常见SLA打印材料进行了比较。在水中一个简单的提取步骤就足以达到材料相对于被测细胞类型的生物相容性。研究了硅水凝胶材料的透氧性，并将其与PDMS、Medicalprint clear（用于医疗产品的商用树脂）和短链水凝胶基树脂进行了比较。作为概念验证，我们展示了一个集成阀门和混合器的3d打印微流体装置。此外，我们展示了一个打印的培养室，用于分析HL-1心肌细胞网络中的信号传播。Ca2+成像用于观察信号在微通道中生长的心脏细胞层中的传播。在打印的腔室中，细胞保持正常的电生理活动。总的来说，生物相容性硅水凝胶材料将是细胞培养和微流控芯片实验室应用中SLA打印的一个进步。
We present a photocurable, biocompatible, and flexible silicone-hydrogel hybrid material for stereolithographic (SLA) printing of biomedical devices. The silicone-hydrogel polymer is similar to mixtures used for contact lenses. It is flexible and stretchable with a Young’s modulus of 78 MPa and a maximum elongation at break of 51%, shows a low degree of swelling (<4% v/v) in water, and can be bonded easily to flat glass substrates via a surface-modification method. Thein vitrocytotoxicity of the material is assessed with a WST-8 cell viability assay using five different cell lines: HT1080, L929, and Hs27 fibroblasts, cardiomyocyte-like HL-1 cells, and neuronal-phenotype PC-12 cells. On this account, the silicone-hydrogel polymer is compared to several other common SLA printing materials used for cell-culture applications and polydimethylsiloxane (PDMS). A simple extraction step in water is sufficient for reaching biocompatibility of the material with respect to the tested cell types. The oxygen permeability of the silicone-hydrogel material is investigated and compared to that of PDMS, Medicalprint clear─a commercial resin for medical products, and a short-chain hydrogel-based resin. As a proof of concept, we demonstrate a 3D-printed microfluidic device with integrated valves and mixers. Furthermore, we show a printed culture chamber for analyzing signal propagation in HL-1 cardiomyocyte cell networks. Ca2+imaging is used to observe the signal propagation through the cardiac cell layers grown in the microchannels. The cells are shown to maintain normal electrophysiological activity within the printed chambers. Overall, the biocompatible silicone-hydrogel material will be an advancement for SLA printing in cell-culture and microfluidic lab-on-a-chip applications.