Isolation of a mouse brain cDNA expressed in developing neuroblasts and mature neurons
Isolation of a mouse brain cDNA expressed in developing neuroblasts and mature neurons
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DOI：
10.1016/s0169-328x(00)00281-3
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发表时间：
2001-01-31
期刊：
影响因子：
--
通讯作者：
Mamalaki, A
中科院分区：
文献类型：
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作者：
Boutou, E;Matsas, R;Mamalaki, A
一个先前未表征的4.5 kb小鼠cDNA克隆，命名为mc7，被分离出来，发现主要在大脑中表达。该cDNA预测了一个1035-bp的开放阅读框，其编码一种345-氨基酸的多肽，该多肽特别富含位于残基80至174区域中的谷氨酸残基。计算分析显示，在其他功能，推定的锌指图案和卷曲螺旋区。在小鼠、大鼠、猪和人类基因组中检测到相应的mc7基因。在小鼠中，mc7 mRNA主要在脑中表达，在肾、肺和脾中表达程度要小得多。在大脑中，早在胚胎第14天就可以食用，而在成人中则保留下来。原位杂交研究表明，mc7 mRNA是广泛的，虽然不均匀，在整个成人大脑的神经元中表达。在小脑皮质的发育原位杂交研究表明，在出生后第5天，MC7 mRNA主要表达在神经母细胞的外部颗粒层和内部颗粒层的发展中的神经元。一些染色也存在于浦肯野细胞中，在出生后第10天变得特别明显，这是树突状树树枝化的时间。在成人小脑皮质的表达主要局限于浦肯野细胞和颗粒神经元在较小程度上。mc7在成神经细胞和发育中的神经元中的早期表达以及其在多种成熟神经元中的保留表明其可能在脑中这些细胞的分化和成熟过程中发挥作用。(C)2001 Elsevier Science B. V.保留所有权利。
A previously uncharacterized 4.5-kb mouse cDNA clone, designated mc7, was isolated and found to be predominantly expressed in brain. This cDNA predicts a 1035-bp open reading frame that encodes for a 345-amino acid polypeptide especially rich in glutamic acid residues located in the region from residues 80 to 174. Computational analysis revealed among other features, putative zinc-finger motifs and coiled-coil regions. The corresponding mc7 gene is detected in mouse, rat, pig and human genomes. In mouse the mc7 mRNA is expressed predominantly in brain and to a much lesser extent in kidney, lung and spleen. In brain it is delectable as early as embryonic day 14 while it is retained in the adult. In situ hybridization studies revealed that mc7 mRNA is widely, albeit unevenly, expressed in neurons throughout the adult brain. Developmental in situ hybridization studies in the cerebellar cortex demonstrated that at postnatal day 5 mc7 mRNA is mainly expressed in neuroblasts of the external granular layer and in developing neurons of the internal granular layer. Some staining is also present in purkinje cells becoming particularly pronounced at postnatal day 10, the time of arborarization of their dendritic tree. In the adult cerebellar cortex expression is mainly confined in purkinje cells and to a lesser extent in granule neurons. The early expression of mc7 in neuroblasts and developing neurons as well as its retention in a wide variety of mature neurons suggest that it may play a role in the process of differentiation and maturation of these cells in the brain. (C) 2001 Elsevier Science B.V. All rights reserved.