Analysis of fumonisin B1 in corn by capillary electrophoresis.
Analysis of fumonisin B1 in corn by capillary electrophoresis.
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DOI：
10.1007/978-1-4899-1379-1_9
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发表时间：
1996
影响因子：
--
通讯作者：
C. Maragos;G. Bennett;J. Richard
中科院分区：
文献类型：
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作者：
C. Maragos;G. Bennett;J. Richard
完整的伏马菌素含有两个丙三羧酸基团，因此可以获得净负电荷。伏马菌素的阴离子性质是用强阴离子交换（SAX）柱净化玉米的广泛使用的方法背后的基础。这种性质也使得伏马菌素能够通过电泳技术分离，而电泳技术至今还没有应用于玉米中伏马菌素的分析。用80/20（v/v）甲醇/水从玉米中提取伏马菌素B1，并用市售亲和柱分离，用异硫氰酸荧光素衍生化，通过毛细管区带电泳-激光诱导荧光检测（CZE-LIF）进行分析。添加0.25 - 5.0 ppm FB 1的玉米的回收率平均为89%（范围为71 - 102%）。在玉米中可检测到0.05ppm的FB 1。对于自然被FB 1污染的玉米，CZE-LIF方法优于已建立的SAX/HPLC和C18/HPLC方法。毛细管电泳可用于定量FB 1在玉米中，与最少的使用有机溶剂，并提供了一个额外的工具，以确认伏马菌素污染。
Intact fumonisins contain two tricarballylic acid groups and can therefore acquire a net negative charge. The anionic nature of the fumonisins is the basis behind the widely used method for cleanup of corn with strong anion exchange (SAX) columns. This property also enables the fumonisins to be separated by electrophoretic techniques which, until now, have not been applied to the analysis of fumonisins in corn. Fumonisin B1, extracted from corn with 80/20 (v/v) methanol/water and isolated with a commercially available affinity column, was derivatized with fluorescein isothiocyanate for analysis by capillary zone electrophoresis with laser-induced fluorescence detection (CZE-LIF). Recoveries from corn fortified with 0.25 to 5.0 ppm FB1averaged 89% (range 71 to 102%). As little as 0.05 ppm FB1could be detected in corn. For corn naturally contaminated with FB1the CZE-LIF method compared favorably to established SAX/HPLC and C18/HPLC methods. Capillary electrophoresis can be used for quantitation of FB1in corn, with minimal use of organic solvents and provides an additional tool for confirming fumonisin contamination.