CHANGES IN CARBOHYDRATE METABOLISM OF MITOGENICALLY STIMULATED HUMAN PERIPHERAL LYMPHOCYTES .2. RELATIVE IMPORTANCE OF GLYCOLYSIS AND OXIDATIVE-PHOSPHORYLATION ON PHYTOHEMAGGLUTININ STIMULATION
CHANGES IN CARBOHYDRATE METABOLISM OF MITOGENICALLY STIMULATED HUMAN PERIPHERAL LYMPHOCYTES .2. RELATIVE IMPORTANCE OF GLYCOLYSIS AND OXIDATIVE-PHOSPHORYLATION ON PHYTOHEMAGGLUTININ STIMULATION
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DOI：
10.1016/0014-4827(73)90561-2
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发表时间：
1973-01-01
影响因子：
3.7
通讯作者：
LOOS, JA
中科院分区：
文献类型：
--
作者：
ROOS, D;LOOS, JA
研究了糖酵解和克雷布斯循环对植物血凝素存在下人血淋巴细胞供能的贡献。在不同的时间收获培养的细胞并重新孵育它们，可以在任何时候测量代谢过程的效力。发现在4小时期间用PHA观察到的碳水化合物代谢的几个参数的增加在1天或3天后更明显。在这种刺激过程中，线粒体或细胞核中底物的氧化与ADP的磷酸化紧密结合。据计算，在正常条件下，15%的ATP是在糖酵解中形成的，85%是在氧化磷酸化中形成的。这些途径中的任一种都可以在短期实验中为PHA刺激提供能量。然而，这两种途径都必须是活跃的长期孵育。
The contribution of glycolysis and Krebs cycle to the energy provision of human blood lymphocytes in the presence of phytohaemagglutinin has been investigated. Harvesting cultured cells at different times and reincubating them allowed measurement of the potency of metabolic processes at any moment. It was found that the increase in several parameters of carbohydrate metabolism as seen with PHA during 4 h, is more pronounced after 1 or 3 days. During this stimulation oxidation of substrates in mitochondria or nuclei remains tightly coupled to phosphorylation of ADP. It was calculated that under normal conditions 15% of the ATP is formed in glycolysis, and 85% in oxidative phosphorylation. Either of these pathways can provide the energy for the PHA stimulation in short-term experiments. However, both pathways must be active for long-term incubations.