Oxygen sensitivity of algal H-2-production
Oxygen sensitivity of algal H-2-production
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DOI：
10.1007/bf02920420
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发表时间：
1997-03-01
影响因子：
3
通讯作者：
Seibert, M
中科院分区：
文献类型：
--
作者：
Ghirardi, ML;Togasaki, RK;Seibert, M
绿色藻类光生H-2利用来自水的光合氧化的电子，并且不需要代谢中间体。然而，藻类氢化酶对O-2非常敏感，这限制了它们在未来商业H-2生产系统中的用途。我们设计了一种实验技术，用于选择O-2耐受性，H-2产生的变种的莱茵衣原体的基础上生存的能力，野生型细胞短（20分钟）暴露于甲硝唑中的存在下，控制浓度的O-2。存活者的数量取决于甲硝唑浓度，光照强度，氢化酶的预诱导，和O-2的存在或不存在。最后，我们证明了一些选定的幸存者，事实上表现出H-2生产能力，是不太敏感的O-2比原来的野生型人口。
Photoproduction of H-2 by green algae utilizes electrons originating from the photosynthetic oxidation of water and does not require metabolic intermediates. However, algal hydrogenases are extremely sensitive to O-2, which limits their usefulness in future commercial H-2-production systems. We designed an experimental technique for the selection of O-2-tolerant, H-2-producing variants of Chlamydomonas reinhardtii based on the ability of wild-type cells to survive a short (20 min) exposure to metronidazole in the presence of controlled concentrations of O-2. The number of survivors depends on the metronidazole concentration, light intensity, preinduction of the hydrogenase, and the presence or absence of O-2. Finally, we demonstrate that some of the selected survivors in fact exhibit H-2-production capacity that is less sensitive to O-2 than the original wild-type population.