Complex and dynamic redistribution of NF-κB signaling intermediates in response to T cell receptor stimulation
Complex and dynamic redistribution of NF-κB signaling intermediates in response to T cell receptor stimulation
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DOI：
10.1073/pnas.0307858100
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发表时间：
2004-01-27
影响因子：
11.1
通讯作者：
Marrack, P
中科院分区：
文献类型：
--
作者：
Schaefer, BC;Kappler, JW;Marrack, P
超分子激活簇的中心区(c-SMAC)是T细胞受体(TCR)富集区，形成于T细胞/抗原提呈细胞(APC)连接处，对抗原刺激做出反应。我们证明了有一个令人惊讶的复杂的重新定位过程，将PKCtheta和Bcl10，这两个TCR激活NF-KB的中间体，带到c-SMAC的细胞质表面。TCR激活导致c-Smac处PKCtheta的丰富，随后Bcl10重新定位到细胞质结构，通常在远离c-Smac的位置。然后，这些Bcl10结构经历进一步的重新定位，在c-SMAC变得丰富。因此，核因子-B-K的TCR激活涉及多种信号中间产物的动态重新定位，在c-Smac附近和远离c-Smac的不同阶段。
The central zone of the supramolecular activation cluster (c-SMAC) is a zone of T cell receptor (TCR) enrichment that forms at a T cell/antigen-presenting cell (APC) junction in response to antigen stimulation. We demonstrate that there is a surprisingly complex relocalization process that brings PKCtheta and Bcl10, two intermediates in TCR activation of NF-KB, to the cytoplasmic face of the c-SMAC. TCR activation causes enrichment of PKCtheta at the c-SMAC, followed by Bcl10 relocalization to punctate cytoplasmic structures, often at sites distant from the c-SMAC. These Bcl10 structures then undergo further relocalization, becoming enriched at the c-SMAC. TCR activation of NF-B-K therefore involves the dynamic relocalization of multiple signaling intermediates, with distinct phases proximal to and distant from the c-SMAC.