EXPRESSION OF MEMBERS OF THE PUTATIVE OLFACTORY RECEPTOR GENE FAMILY IN MAMMALIAN GERM-CELLS
EXPRESSION OF MEMBERS OF THE PUTATIVE OLFACTORY RECEPTOR GENE FAMILY IN MAMMALIAN GERM-CELLS
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DOI：
10.1038/355453a0
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发表时间：
1992-01-30
期刊：
影响因子：
64.8
通讯作者：
VASSART, G
中科院分区：
文献类型：
--
作者：
PARMENTIER, M;LIBERT, F;VASSART, G
通过同源克隆和低严格率聚合酶链式反应1，2分离了一系列编码G蛋白偶联受体新成员的基因组和互补DNA克隆1，2。在未知受体(孤儿受体)中，人类基因组克隆(HGMP07)的特征是其转录产物存在于睾丸中，并且属于一个具有广泛序列相似性的基因亚家族。序列比较表明，该基因亚家族是最近克隆的大鼠嗅觉受体家族的人类亚家族成员。Northern blotting进一步证明生殖细胞中存在嗅觉受体转录本。我们的发现表明，一个共同的受体基因家族编码了嗅觉受体和精子细胞受体，它们可能参与受精过程中的趋化作用。
A SERIES of genomic and complementary DNA clones encoding new putative members of G protein-coupled receptors were isolated using homology cloning and low-stringency polymerase chain reaction 1,2. Among the unidentified receptors ('orphan receptors'), a human genomic clone (HGMP07) was characterized by the presence of its transcripts in the testis and by its belonging to a large subfamily of genes sharing extensive sequence similarities. Sequence comparison demonstrated that this gene subfamily is the human counterpart of the putative rat olfactory receptors cloned recently 3. Another 48 members of the family were cloned. Northern blotting further demonstrated the presence of olfactory receptor transcripts in germ cells. Our finding suggests that a common receptor gene family encodes olfactory receptors and sperm cell receptors that could be involved in chemotaxis during fertilization.