SEQUENCE DETERMINATION AND COMPARISON OF THE EXFOLIATIVE TOXIN-A AND TOXIN-B GENES FROM STAPHYLOCOCCUS-AUREUS
SEQUENCE DETERMINATION AND COMPARISON OF THE EXFOLIATIVE TOXIN-A AND TOXIN-B GENES FROM STAPHYLOCOCCUS-AUREUS
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DOI：
10.1128/jb.169.9.3904-3909.1987
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发表时间：
1987-09-01
影响因子：
3.2
通讯作者：
IANDOLO, JJ
中科院分区：
文献类型：
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作者：
LEE, CY;SCHMIDT, JJ;IANDOLO, JJ
将编码金黄色葡萄球菌的脱落毒素A基因（eta）的DNA克隆到噬菌体λ gt 11中，随后克隆到质粒pLI 50中染色体的1，391-碱基对DNA片段上。脱落毒素A在大肠杆菌遗传背景中表达，长度与从培养基中纯化的毒素相似，并且在动物测定中具有生物活性。测定了含有该基因的DNA片段的核苷酸序列。从核苷酸序列推导的蛋白质是280个氨基酸的多肽。成熟蛋白由242个氨基酸组成。测定了脱落毒素B基因的DNA序列。氨基酸序列的校正表明，脱落毒素B的氨基酸序列与化学序列数据雅阁。
The DNA encoding the exfoliative toxin A gene (eta) of Staphylococcus aureus was cloned into bacteriophage .lambda.gt11 and subsequently into plasmid pLI50 on a 1,391-base-pair DNA fragment of the chromosome. Exofoliative toxin A is expressed in the Escherichia coli genetic background, is similar in length to the toxin purified from culture medium, and is biologically active in an animal assay. The nucleotide sequence of the DNA fragment containing the gene was determined. The protein deduced from the nucleotide sequence is a polypeptide of 280 amino acids. The mature protein is 242 amino acids. The DNA sequence of the exfoliative toxin B gene was also determined. Corrections indicate that the amino acid sequence of exfoliative toxin B is in accord with chemical sequence data.