Propulsive appliance stimulates, the synthesis of insulin-like growth factors I and II in the mandibular condylar cartilage of young rats
Propulsive appliance stimulates, the synthesis of insulin-like growth factors I and II in the mandibular condylar cartilage of young rats
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DOI：
10.1016/s0003-9969(03)00128-6
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发表时间：
2003-09-01
影响因子：
3
通讯作者：
Kimura, ET
中科院分区：
文献类型：
--
作者：
Hajjar, D;Santos, MF;Kimura, ET
功能性矫形矫治器部分是通过对髁突软骨施加间接的机械刺激，调节生长和口面部结构的适应来矫正牙齿错牙合。然而，这种疗法的生物学反应的确切性质还不是很清楚。胰岛素样生长因子I和II(IGF-I和IGF-II)是包括软骨在内的多种组织生长和分化过程中的重要局部因子。本研究的目的是验证幼龄Wistar大鼠下颌前伸矫治器使用3、5、7、9、11、13和15天后，髁突软骨中IGF-I和IGF-II的基因和蛋白表达。为此，脱钙和石蜡包埋的髁突的矢状面切片被提交给免疫组织化学和原位杂交。对照组和治疗组大鼠IGF-I和IGF-II的表达随着发育年龄的增加而增加。治疗9天后，佩戴推进器的动物的这两种多肽的阳性率甚至增加得更多，这与年龄匹配的对照组明显不同。两种IGF的表达模式相似，但IGF-I标记较强。此外，这两种多肽的表达增强与增殖细胞核抗原(增殖细胞核抗原)阳性的增殖细胞标志是平行的。IGF-I和IGF-II在下颌骨软骨中的表达随推进矫治器的作用而变化，提示这两种多肽都参与了下颌骨的适应。(C)2003爱思唯尔有限公司。保留所有权利。
Functional orthopedic appliances correct dental malocclusion partially by exerting indirect mechanical stimulus on the condylar cartilage, modulating growth and the adaptation of orofacial structures. However, the exact nature of the biological responses to this therapy is not well understood. Insulin-like growth factors I and II (IGF-I and IGF-II) are important local factors during growth and differentiation of several tissues, including cartilage. The aim of this study was to verify the mRNA and protein expression of IGF-I and IGF-II in the condylar cartilage of young mate Wistar rats that used a mandibular propulsive appliance for 3, 5, 7, 9, 11, 13 or 15 days. For this purpose, sagittal sections of decalcified and paraffin-embedded condyles were submitted to immunohistochemistry and in situ hybridization. IGF-I and IGF-II expression increased with developmental age in the control and treated rats. After 9 days of treatment the positivity for both peptides in the animals that wore the propulsive appliance increased even more, expressively different from the age-matched controls. The expression patterns of both IGFs were similar, although IGF-I labelling was stronger. Furthermore, the enhanced expression of both peptides was in parallel with the proliferating cell nuclear antigen (PCNA) positivity, a proliferation cell marker. The modulation of IGF-I and IGF-II expression in the condylar cartilage in response to the propulsive appliance suggests that both peptides are involved in the mandibular adaptation during this therapy. (C) 2003 Elsevier Ltd. All rights reserved.