Design and use of fluorescent fusion proteins in cell biology.
Design and use of fluorescent fusion proteins in cell biology.
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DOI：
10.1002/0471143030.cb2104s27
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发表时间：
2005-07-01
影响因子：
--
通讯作者：
Snapp, Erik
中科院分区：
文献类型：
--
作者：
Snapp, Erik
本单元描述了设计功能性荧光融合蛋白结构的策略。这些结构可以被用作细胞环境、蛋白质动力学、蛋白质生命史、蛋白质结合伙伴和活细胞中的标记的探针。讨论了目前可用的许多荧光蛋白的性质和用途。此外，还提供了替代方法和故障排除指南。
This unit describes strategies for designing functional fluorescent fusion protein constructs. Such constructs can be exploited as probes of cellular environments, protein dynamics, protein life histories, protein binding partners, and markers in living cells. The properties and uses of many currently available fluorescent proteins are discussed. In addition, alternative approaches and troubleshooting guidelines are provided.