Hydropathic characteristics of adenovirus hexons
Hydropathic characteristics of adenovirus hexons
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DOI：
10.1007/s007050050162
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发表时间：
1997-01-01
影响因子：
2.7
通讯作者：
Wadell, G
中科院分区：
文献类型：
--
作者：
Li, QG;Lindman, K;Wadell, G
测定了腺病毒7型六邻体基因的完整核苷酸序列和预测的氨基酸序列。分析了来自人腺病毒2、3、4、5、7、12、16、40、41和48型、牛腺病毒3型、鼠腺病毒I型和禽腺病毒I型和10型的六邻体蛋白的亲水性。密码子第二位嘌呤和嘧啶的存在分别与亲水性和疏水性相关。比较8种鹭的亲水性图，发现表面分布有7个高变区。高变区的大部分表现出亲水性。在亲水和疏水区域上积累的表面电荷的强度与不同腺病毒类型的组织嗜性相关。对腺病毒Herons的密码子使用分析表明，在同义密码子中，第三位带有胞苷的密码子在很大程度上被优先使用。对14个六邻体蛋白的核苷酸和氨基酸序列对距离分析和系统发育树分析表明，B、D和E亚属的成员关系密切，尤其是Ad4和Ad16，A亚属与F亚属关系密切。
The complete nucleotide sequence and the predicted amino acid sequence of the adenovirus type 7 hexon gene were determined. The hydropathy of the hexon proteins from human adenovirus types 2, 3, 4, 5, 7, 12, 16, 40, 41, and 48, bovine adenovirus type 3, murine adenovirus type I, and avian adenovirus types I and 10 was analysed. The presence of purines and pyrimidines in the second position of the codons was correlated to hydrophilicity and hydrophobicity, respectively. Comparison of the hydrophilicity plots of eight herons showed seven hypervariable regions to be distributed on the surface. A large portion of the hypervariable regions manifests hydrophilicity. The strength of the surface charge accumulated on the hydrophilic and hydrophobic regions correlated to the tissue tropism of the different adenovirus types. Analysis of codon usage for adenovirus herons showed that among synonymous codons those with cytidine in the third position were preferably used to a great extent. Analysis of the nucleotide and amino acid sequence pair distances and the phylogenetic tree of 14 hexon proteins showed members of subgenera B, D and E to be closely related, especially Ad4 and Ad16, and subgenus A to be closely related to subgenus F.