Basic image analysis and manipulation in ImageJ.
Basic image analysis and manipulation in ImageJ.
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DOI：
10.1002/0471142727.mb1415s102
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发表时间：
2013-01-01
影响因子：
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通讯作者：
Hartig, Sean M
中科院分区：
文献类型：
--
作者：
Hartig, Sean M
图像分析方法已被开发，以提供定量评估的显微镜数据。在本单元中，概述了图像分析的基本方面，包括软件安装，数据导入，图像处理功能，以及可用于使用ImageJ从显微镜数据中提取信息的分析工具。逐步的协议，用于分析对象的荧光图像和提取信息的双色组织图像收集的明场显微镜。
Image analysis methods have been developed to provide quantitative assessment of microscopy data. In this unit, basic aspects of image analysis are outlined, including software installation, data import, image processing functions, and analytical tools that can be used to extract information from microscopy data using ImageJ. Step-by-step protocols for analyzing objects in a fluorescence image and extracting information from two-color tissue images collected by bright-field microscopy are included.