The Inverted Heart Model for Interstitial Transudate Collection from the Isolated Rat Heart

The Inverted Heart Model for Interstitial Transudate Collection from the Isolated Rat Heart
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DOI：
10.3791/55849
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发表时间：
2017
期刊：
Journal of Visualized Experiments (JoVE)
影响因子：
--
通讯作者：
Jürgen Schrader
Jürgen Schrader
中科院分区：
--
文献类型：
--
作者：
Kezhe Tan;Zhaoping Ding;Bodo Steckel;Sonja Hartwig;Stefan Lehr;Xiaoming Deng;Jürgen Schrader

文献摘要

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This protocol describes a method to collect cardiac interstitial fluid from the isolated, perfused rat heart. To physically separate interstitial transudate from coronary venous effluent perfusate, the Langendorff perfused heart is inverted, and the transudate (interstitial fluid) formed on the cardiac surface is collected using a soft latex cap.