Albumin-bound docosahexaenoic acid and collagen-induced human platelet reactivity
Albumin-bound docosahexaenoic acid and collagen-induced human platelet reactivity
复制标题
白蛋白结合的二十二碳六烯酸和胶原诱导的人血小板反应性
作者：
D. Gaudette;B. Holub
采用体外实验系统模拟各种血浆非酯化(多不饱和)脂肪酸对血小板功能和代谢的影响。2 0μM白蛋白结合的二十二碳六烯酸(2 2：6N−3)预先孵育2min，可显著抑制亚极量(1.8mgg/ml)胶原刺激诱导的人血小板聚集，而其他脂肪酸对此无明显影响。二十碳五烯酸(20：5N−3)、11，14，17-二十碳三烯酸(20：3N−3)、二高γ亚麻酸(20：3N−6)和DHA可适度抑制磷脂酶C(PA)的形成，但不能被花生四烯酸(20：4N−6)抑制，这种抑制磷脂酶C活性的作用不能解释DHA对血小板聚集的不同作用。用[~H]12-羟基-5，8，10-十七碳三烯酸(HHT)的形成来评价血栓素A2(TXA2)的生成减少可能是20μM DHA抑制胶原诱导的聚集的原因之一。令人惊讶的是，与40μM白蛋白结合的DHA预先孵育，尽管对胶原诱导的聚集有更大的抑制作用，但对羟色胺的形成影响较小。经3分钟胶原刺激后[~3H]前列腺素D2(PGD2)水平轻微但显著升高可能是40μM DHA具有更强的抗聚集作用的原因之一。结论：补充鱼油后，血浆非酯化DHA的升高可能是导致血小板活化抑制和代谢改变的原因之一。
Anin vitro system designed to mimic the effect of various plasma nonesterified (polyunsaturated) fatty acids on platelet function and metabolism was employed. Human platelet aggregation induced by submaximal (1.8 μg/ml) collagen stimulation was significantly inhibited by 2 min preincubation with 20 μM albumin-bound docosahexaenoic acid (22∶6n−3) (DHA), but not by the other fatty acids tested. [3H]Phosphatidic acid (PA) formation, an indicator of phospholipase C activation following platelet stimulation, was moderately inhibited by eicosapentaenoic acid (20∶5n−3), 11,14,17-eicosatrienoic acid (20∶3n−3), dihomo-γ-linolenic acid (20∶3n−6), as well as DHA, but not by arachidonic acid (20∶4n−6); this inhibition of phospholipase C activation could not explain the differential effect of DHA on platelet aggregation. The decreased production of thromboxane A2 (TxA2), as assessed by [3H]12-hydroxy-5,8,10-heptadecatrienoic acid (HHT) formation, may account for the inhibition of collagen-induced aggregation by 20 μM DHA. Surprisingly, preincubation with 40 μM albumin-bound DHA, even though resulting in greater inhibition of collagen-induced aggregation, had less impact on HHT formation. A small but significant increase in [3H]prostaglandin D2 (PGD2) levels following 3-min collagen stimulation may have contributed to the greater antiaggregatory effect of 40 μM DHA. It is concluded that increased plasma nonesterified DHA may contribute to the dampened platelet activation and altered metabolism following fish oil supplementation of the diet.
影响因子：
--
作者：
A. Spector
通讯作者：
A. Spector
二十二碳六烯酸（DHA）对花生四烯酸代谢和血小板功能的影响。
DOI：
10.1016/0006-291x(83)91235-4
发表时间：
1983
影响因子：
3.1
作者：
Rao,GH;Radha,E;White,JG
通讯作者：
White,JG
二酰基甘油导致血小板致密管状系统释放 Ca：与肌醇 1,4,5-三磷酸引起的 Ca 释放进行比较。
DOI：
10.1016/0006-291x(87)90444-x
发表时间：
1987
影响因子：
3.1
作者：
Brass,LF;Laposata,M
通讯作者：
Laposata,M