Expression of the anti-metastatic effect induced by Juzen-taiho-to is based on the content of Shimotsu-to constituents
Expression of the anti-metastatic effect induced by Juzen-taiho-to is based on the content of Shimotsu-to constituents
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DOI：
10.1248/bpb.21.761
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发表时间：
1998-07-01
影响因子：
2
通讯作者：
Saika, I
中科院分区：
文献类型：
--
作者：
Onishi, Y;Yamaura, T;Saika, I
本实验研究了日本汉布中草药橘子参太和素及其相关制剂对体内肿瘤细胞肝、肺转移的抑制作用。肿瘤接种前口服橘子参太和素7d可显著降低结肠癌26-L5肝癌细胞肝转移菌落数量，并在4 ~ 40 mg/d范围内呈剂量依赖性减轻肝脏重量的增加。在肿瘤接种前同一时期口服，对B16-BL6黑色素瘤细胞的肺转移也有显著抑制作用。橘子参太和素最初由下子素和石kunshito方的8种生药以及肉桂皮和黄芪2种生药组成。在肿瘤接种前口服下田-to和Juzen-taiho-to 7 d，与对照组相比，转移瘤数量和肝脏重量显著减少，而石kunshi -to没有这种抑制作用，含有下田-to四种成分的Unsei-in也有抑制肝脏转移的活性。Toki-shakuyaku-san和Ninjin-yoei-to分别含有除地黄和蛇床根外的所有下素-to成分，其抗转移作用不显著。含石胆土成分的立胆石土和忍仁约宜土对肝转移的抑制作用无明显影响。肿瘤接种前治疗虎竹-太和素对肝转移也有显著抑制作用，其抑制机制可能不同于朱竹-太和素。这些结果表明，橘子仁-太合-的抗转移作用与其下子-衍生成分有关。
We investigated the inhibitory effect of oral administration of Juzen-taiho-to, a Kampo Japanese herbal medicine, and its related formulations on the experimental liver and lung metastasis of tumor cells in vivo. Oral administration of Juzen-taiho-to for 7d before tumor inoculation significantly reduced the number of liver metastatic colonies of colon 26-L5 carcinoma cells and attenuated the increase of liver weight in a dose-dependent manner ranging from 4 to 40 mg/d. Its oral administration for this same period before tumor inoculation also significantly inhibited lung metastasis of B16-BL6 melanoma cells, Juzen-taiho-to originally consisted of 8 crude drugs derived from Shimotsu-to and Shikunshi-to prescriptions together with two crude drugs (Cinnamomi Cortex and Astragali Radix). Oral administration of Shimotsu-to as well as Juzen-taiho-to for 7 d before tumor inoculation resulted in a significant reduction in the number of metastatic felonies and the liver weight as compared with the control, whereas Shikunshi-to did not exhibit such an inhibitory effect, Unsei-in containing four Shimotsu-to constituents was also active in inhibiting liver metastasis. Toki-shakuyaku-san and Ninjin-yoei-to, which include all Shimotsu-to constituents except Rehmanniae Radix and Cnidii Rhizoma, respectively, did not show a significant anti-metastatic effect. Rikkunshi-to and Ninjin-yoei-to, which contain Shikunshi-to constituents, did not affect the inhibition of liver metastasis. Hochu-ekki-to treatment before tumor inoculation also led to a significant inhibition of liver metastasis, probably through an inhibitory mechanism different from Juzen-taiho-to. These results suggest that the anti-metastatic effect of Juzen-taiho-to is partly associated with its Shimotsu-to-derived constituents.