The role of putrescine in the regulation of proteins and fatty acids of thylakoid membranes under salt stress.
The role of putrescine in the regulation of proteins and fatty acids of thylakoid membranes under salt stress.
复制标题
腐胺在盐胁迫下调节类囊体膜蛋白质和脂肪酸中的作用
DOI：
10.1038/srep14390
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发表时间：
2015-10-05
影响因子：
4.6
通讯作者：
Guo S
中科院分区：
文献类型：
--
作者：
Shu S;Yuan Y;Chen J;Sun J;Zhang W;Tang Y;Zhong M;Guo S
多胺可以通过调节光合效率来缓解盐胁迫对植物生长的抑制作用。然而，很少有资料可以解释的具体机制，多胺的光合机构的耐盐性的贡献。研究了腐胺对盐胁迫下黄瓜幼苗光合机构的影响。结果表明，NaCl胁迫导致黄瓜叶绿体受到严重的离子毒害和氧化胁迫。此外，盐度引起类囊体膜饱和脂肪酸含量的显着增加。改变了不饱和脂肪酸的含量，从而减轻了类囊体基粒层的解体，减少了类囊体膜上的质体数量。BN-PAGE显示Put上调NaCl胁迫下类囊体膜ATP合成酶、CP 47、D1、Qb和psbA蛋白的表达，下调CP 24、D2和LHCII III型蛋白的表达。使用基因表达的qRT-PCR分析来比较10种候选蛋白质的转录和蛋白质积累。对于这些蛋白质中的五种，诱导的转录物积累与诱导的蛋白质积累的模式一致。我们的研究结果表明，把调节蛋白质的表达在转录和翻译水平增加内源多胺水平的类囊体膜，这可能稳定光合机构在盐胁迫下。
Polyamines can alleviate the inhibitory effects of salinity on plant growth by regulating photosynthetic efficiency. However, little information is available to explain the specific mechanisms underlying the contribution of polyamines to salt tolerance of the photosynthetic apparatus. Here, we investigated the role of putrescine (Put) on the photosynthetic apparatus of cucumber seedlings under salt stress. We found that NaCl stress resulted in severe ion toxicity and oxidative stress in cucumber chloroplasts. In addition, salinity caused a significant increase in the saturated fatty acid contents of thylakoid membranes. Put altered unsaturated fatty acid content, thereby alleviating the disintegration of thylakoid grana lamellae and reducing the number of plastoglobuli in thylakoid membranes. BN-PAGE revealed Put up-regulated the expression of ATP synthase, CP47, D1, Qb and psbA proteins and down-regulated CP24, D2 and LHCII type III in NaCl-stressed thylakoid membranes. qRT-PCR analysis of gene expression was used to compare transcript and protein accumulation among 10 candidate proteins. For five of these proteins, induced transcript accumulation was consistent with the pattern of induced protein accumulation. Our results suggest that Put regulates protein expression at transcriptional and translational levels by increasing endogenous polyamines levels in thylakoid membranes, which may stabilise photosynthetic apparatus under salt stress.