Proteomic Analysis of RBP4/Vitamin A in Children with Cleft Lip and/or Palate
Proteomic Analysis of RBP4/Vitamin A in Children with Cleft Lip and/or Palate
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DOI：
10.1177/0022034514530397
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发表时间：
2014-06-01
影响因子：
7.6
通讯作者：
Chen, F.
中科院分区：
文献类型：
--
作者：
Zhang, J.;Zhou, S.;Chen, F.
唇腭裂是最常见的先天性颅面畸形之一。非综合征型CLP（NSCLP）是一种受遗传和环境因素相互作用影响的多因素疾病。然而，很少有研究报告的发育或代谢状态的婴儿与出生后的NSCLP。在我们的研究中，我们试图确定和评估血清蛋白质谱的差异表达，在NSCLP儿童和未受影响的婴儿。因此，首先使用“鸟枪蛋白质组学”方法分析13名2至3.5岁的NSCLP儿童和10名对照儿童的血浆蛋白质组。在血清样品中总共鉴定了300多种蛋白质。通过基因本体（GO）分析，我们发现了许多可能与非小细胞肺癌相关的差异表达蛋白，包括参与脂蛋白代谢、胰岛素样生长因子相关过程等，尤其是参与视黄醇转运的蛋白。视黄醇结合蛋白4（RBP4），一种视黄醇转运蛋白，在NSCLP组中显著降低。因此，通过高效液相色谱法（HPLC）进一步测定血清维生素A水平。维生素A浓度也存在显著差异（p <0.01），与RBP4的趋势一致。我们的研究结果表明，RBP4和维生素A水平的降低与新生儿NSCLP有关，因此应该得到更多的关注。这些结果还表明，在早期阶段补充维生素A可能是必要的。
Cleft of the lip and/or palate (CLP) is one of the most common congenital craniofacial defects. Non-syndromic CLP (NSCLP) is a multifactorial disease influenced by the interaction of genetic and environmental factors. However, there are few studies reporting on the developmental or metabolic status of babies with NSCLP after birth. In our study, we sought to identify and evaluate the differential expression of serum protein profiles in NSCLP children and unaffected babies. Thus, a 'shotgun proteomics' approach was first used to analyze the plasma proteome of 13 children with NSCLP and 10 control children, aged 2 to 3.5 years. In total, more than 300 proteins were identified in the serum sample. With gene ontology (GO) analysis, we detected many differentially expressed proteins that could be related to NSCLP, including those involved in lipoprotein metabolism, insulin-like growth-factor-related processes, and so on, especially the proteins involved in retinol transport. Retinol binding protein 4 (RBP4), one protein of the retinol transport category, was significantly decreased in the NSCLP group. Thus, serum vitamin A levels were further determined by high-performance liquid chromatography (HPLC). A significant difference (p < .01) was also found in vitamin A concentrations, consistent with the trend of RBP4. Our results indicated that reduced levels of RBP4 and vitamin A were related to newborns with NSCLP and should thus receive more attention. These results also suggest that vitamin A supplementation might be necessary at an early stage.