Chondrogenic differentiation of human mesenchymal stem cells cultured in a cobweb-like biodegradable scaffold
Chondrogenic differentiation of human mesenchymal stem cells cultured in a cobweb-like biodegradable scaffold
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DOI：
10.1016/j.bbrc.2004.07.071
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发表时间：
2004-09-10
影响因子：
3.1
通讯作者：
Tateishi, T
中科院分区：
文献类型：
--
作者：
Chen, GP;Liu, DC;Tateishi, T
将人间充质干细胞（MSCs）在含tgf - β 3的无血清DMEM中体外培养1-10周，这种网状生物可降解聚合物支架是聚dl -乳酸-共乙醇酸-胶原混合网状结构。细胞粘附在杂交网状结构上，分布均匀，增殖填充支架内的空间。细胞表达编码I型胶原蛋白基因的能力下降，而表达II型胶原蛋白和聚集蛋白的能力增加。HE染色组织学检查显示细胞早期呈成纤维细胞形态，培养4周后呈圆形。红素O和甲苯胺蓝染色软骨基质呈阳性。抗II型胶原蛋白和抗软骨蛋白多糖免疫染色显示细胞周围检测到II型胶原蛋白和软骨蛋白多糖。此外，在细胞周围检测到均匀分布的软骨细胞外基质。这些结果提示在杂交网状结构中间充质干细胞具有成软骨分化的作用。plga -胶原杂交网状物使间充质干细胞聚集，为间充质干细胞的软骨分化提供了促进微环境。(C) 2004爱思唯尔公司版权所有。
Human mesenchymal stem cells (MSCs) were cultured in vitro in a cobweb-like biodegradable polymer scaffold: a poly(DL-lactic-co-glycolic acid)-collagen hybrid mesh in serum-free DMEM containing TGF-beta3 for 1-10 weeks. The cells adhered to the hybrid mesh, distributed evenly, and proliferated to fill the spaces in the scaffold. The ability of the cells to express gene encoding type I collagen decreased, whereas its ability to express type II collagen and aggrecan increased. Histological examination by HE staining indicated that the cells showed fibroblast morphology at the early stage and became round after culture for 4 weeks. The cartilaginous matrices were positively stained by safranin O and toluidine blue. Immunostaining with anti-type II collagen and anti-cartilage proteoglycan showed that type II collagen and cartilage proteoglycan were detected around the cells. In addition, a homogeneous distribution of cartilaginous extracellular matrices was detected around the cells. These results suggest the chondrogenic differentiation of the mesenchymal stem cells in the hybrid mesh. The PLGA-collagen hybrid mesh enabled the aggregation of mesenchymal stem cells and provided a promotive microenvironment for the chondrogenic differentiation of the MSCs. (C) 2004 Elsevier Inc. All rights reserved.