Agarose–gelatin conjugate membrane enhances proliferation of adherent cells enclosed in hollow-core microcapsules
Agarose–gelatin conjugate membrane enhances proliferation of adherent cells enclosed in hollow-core microcapsules
复制标题
琼脂糖-明胶缀合物膜增强空心微胶囊中贴壁细胞的增殖
DOI：
10.1163/156856208784613587
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发表时间：
2008
期刊：
影响因子：
--
通讯作者：
K. Kawakami
中科院分区：
文献类型：
--
作者：
S. Sakai;I. Hashimoto;K. Kawakami
控制中空微胶囊中细胞的生长是该装置在生物医学和生物制药领域实际应用的重要问题。在这项研究中，我们开发了中空微胶囊与细胞粘附琼脂糖明胶共轭物（琼脂-锗）凝胶膜，以增强贴壁细胞生长。我们封闭贴壁猫肾细胞在这些微胶囊中，并比较其生长曲线和行为与细胞在微胶囊与未改性琼脂糖膜。细胞生长接近。2-倍更快的微胶囊与琼脂-锗膜比那些未改性琼脂糖膜。细胞骨架的荧光观察清楚地表明，封闭的细胞粘附和蔓延的内表面上的琼脂-Ge膜，但不是在未改性的琼脂糖膜。最大细胞密度估计的基础上的细胞线粒体的活动是独立的细胞膜的厚度。对于两种类型的微胶囊，每种载体的线粒体活性相似。这些结果表明，从细胞粘附材料的微胶囊膜的建设是有效的，以提高这些装置中的细胞生长。
Controlling growth of cells enclosed in hollow-core microcapsules is an important issue for the practical use of the device in biomedical and biopharmaceutical fields. In this study, we developed hollow-core microcapsules with a cell-adhesive agarose–gelatin conjugate (Aga–Ge) gel membrane for enhancement of adherent cell growth. We enclosed adherent feline kidney cells in these microcapsules and compared their growth profile and behavior with cells in microcapsules with an unmodified agarose membrane. The cells grew approx. 2-fold faster in microcapsules with the Aga–Ge membrane than in those with the unmodified agarose membrane. Fluorescence observation of the cellular skeleton clearly revealed that the enclosed cells adhered and spread on the inner surface of the Aga–Ge membrane but not on the unmodified agarose membrane. The maximum cell densities estimated on the basis of the cellular mitochondrial activities were independent of the cellular adhesiveness of the membrane. The mitochondrial activities per vehicle were similar for the two types of microcapsules. These results demonstrate that construction of microcapsule membranes from cell-adhesive materials is effective for enhancing cellular growth in these devices.