Plasma luteinizing hormone dynamics in pregnant mare serum-induced ovulators as measured by radioimmunoassay: influence of testosterone.
Plasma luteinizing hormone dynamics in pregnant mare serum-induced ovulators as measured by radioimmunoassay: influence of testosterone.
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通过放射免疫测定测量怀孕母马血清诱导排卵的血浆黄体生成激素动态：睾酮的影响。
DOI：
10.1210/endo-88-5-1131
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发表时间：
1971
期刊：
影响因子：
4.8
通讯作者：
D. Schalch
中科院分区：
文献类型：
--
作者：
D. L. Klawon;S. Sorrentino;D. Schalch
孕马血清（PMS）已被用于诱导未成熟雌性大鼠排卵。皮下给予25 IU PMS后，60- 87%的动物排卵（5-44个卵子/动物），在2个单独的实验中，发现“排卵前”血浆LH水平分别为422±76和497±72（平均值+ SE）ng/ml（NIAMD-大鼠LH-RP 1），而“排卵后”水平分别为168±11和154 ±11 ng/ml。排卵前血浆LH（ΔLH）升高与个体大鼠排出的卵子数之间不存在相关性。在给予12.5和25 IU PMS的大鼠中，发现平均ΔLH和排卵大鼠排出的卵子数量均存在剂量反应关系。丙酸替卡松（500 μg/d，连用4天）完全抑制LH的释放和促排卵作用。这些数据表明，在PMS诱导的排卵者中，排卵前期血浆LH有一致但可变或异步的升高，睾酮通过抑制这种“排卵前”升高来阻断排卵。这...
Pregnant mare serum (PMS) has been used to induce ovulation in immature female rats. After the subcutaneous administration of 25 IU of PMS, 60-87 % of the animals ovulated (5-44 ova/animal), and “pre-ovulatory” plasma LH levels in 2 separate experiments were found to be 422±76 and 497±72 (mean + SE) ng/ml (NIAMD-Rat LH-RP1) compared to “post-ovulatory” levels of 168±11 and 154 ±11 ng/ml, respectively. No correlation existed between the “pre-ovulatory” rise in plasma LH (ΔLH) and the number of ova shed by individual rats. In rats given 12.5 vs. 25 IU of PMS, a dose response relationship was found both in the mean ΔLH and number of ova shed by ovulating rats. Testosterone propionate (500 μg/day for 4 days) completely suppressed the release of LH and the induction of ovulation. These data indicate that there is a consistent though variable or asynchronous rise in plasma LH in the pre-ovulatory period in PMS-induced ovulators and that testosterone blocks ovulation by suppressing this “pre-ovulatory” rise. Thi...