Comparative Transcriptome Analysis Reveals Hormone Signal Transduction and Sucrose Metabolism Related Genes Involved in the Regulation of Anther Dehiscence in Photo-Thermo-Sensitive Genic Male Sterile Wheat.
Comparative Transcriptome Analysis Reveals Hormone Signal Transduction and Sucrose Metabolism Related Genes Involved in the Regulation of Anther Dehiscence in Photo-Thermo-Sensitive Genic Male Sterile Wheat.
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比较转录组分析揭示了参与光热敏感基因雄性不育小麦花药开裂调节的激素信号转导和蔗糖代谢相关基因。
DOI：
10.3390/biom12081149
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发表时间：
2022-08-20
期刊：
影响因子：
5.5
通讯作者：
中科院分区：
文献类型：
--
作者：
花药开裂是释放花粉的重要过程，也是授粉过程中的关键事件。在小麦光温敏核不育系(PTGMS)中，由于在不育条件下，花药裂开期间花药不能裂开，因此不能从花药中释放花粉。本研究利用RNA测序技术对小麦光温敏核不育系BS366在可育和不育条件下的花药开裂过程中的转录组进行了分析，以探讨其作用机制。我们鉴定了6306个差异表达基因(Deg)。加权基因共表达网络分析(WGCNA)和KEGG分析表明DEGS主要与“激素信号转导途径”和“淀粉和蔗糖代谢”有关。我们分别鉴定了35和23个与激素信号转导和蔗糖代谢相关的基因。与常规小麦京411相比，BS366在不育条件下，在花药裂开的三个时期，激素(JA、IAA、BR、ABA、GA3)和蔗糖的含量发生了一定的变化。我们用qRT-PCR验证了激素信号通路和淀粉和蔗糖代谢通路中一些关键点的表达水平。结果表明，激素信号途径和淀粉、蔗糖代谢途径的关键点在不同条件下的表达模式不同，提示这些基因可能参与了BS366花药开裂的调控。最后，我们建立了一个假说模型来揭示激素和蔗糖对花药开裂的调控途径。这些信息为研究小麦花药开裂的分子机制和改良小麦杂交育种提供了新的线索。
Anther dehiscence is an important process to release pollen and then is a critical event in pollination. In the wheat photo-thermo-sensitive genic male sterility (PTGMS) line, pollen cannot release from anther since the anther cannot dehisce during anther dehiscence stage in a sterile condition. In this study, we carried out RNA-sequencing to analyze the transcriptome of one wheat PTGMS line BS366 during anther dehiscence under fertile and sterile conditions to explore the mechanism. We identified 6306 differentially expressed genes (DEGs). Weighted gene co-expression network analysis (WGCNA) and KEGG analysis showed that DEGs were mainly related to “hormone signal transduction pathway” and “starch and sucrose metabolism”. We identified 35 and 23 DEGs related hormone signal transduction and sucrose metabolism, respectively. Compared with conventional wheat Jing411, there were some changes in the contents of hormones, including JA, IAA, BR, ABA and GA3, and sucrose, during three anther dehiscence stages in the sterile condition in BS366. We performed qRT-PCR to verify the expression levels of some critical DEGs of the hormone signaling pathway and the starch and sucrose metabolism pathway. The results showed disparate expression patterns of the critical DEGs of the hormone signaling pathway and the starch and sucrose metabolism pathway in different conditions, suggesting these genes may be involved in the regulation of the anther dehiscence in BS366. Finally, we conducted a hypothesis model to reveal the regulation pathway of hormones and sucrose on anther dehiscence. The information provided new clues to the molecular mechanisms of anther dehiscence in wheat and improved wheat hybrid breeding.
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影响因子：
3
作者：
Langfelder P;Horvath S
通讯作者：
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影响因子：
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影响因子：
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作者：
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通讯作者：
Wan, Xiangyuan
影响因子：
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