OBSERVATIONS OF VASCULARIZATION IN THE SPINAL-CORD OF MOUSE EMBRYOS, WITH SPECIAL REFERENCE TO DEVELOPMENT OF BOUNDARY MEMBRANES AND PERIVASCULAR SPACES
OBSERVATIONS OF VASCULARIZATION IN THE SPINAL-CORD OF MOUSE EMBRYOS, WITH SPECIAL REFERENCE TO DEVELOPMENT OF BOUNDARY MEMBRANES AND PERIVASCULAR SPACES
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DOI：
10.1002/ar.1092210212
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发表时间：
1988-06-01
影响因子：
--
通讯作者：
OGAWA, R
中科院分区：
文献类型：
--
作者：
NAKAO, T;ISHIZAWA, A;OGAWA, R
用图形重建技术和电子显微镜研究了小鼠脊髓血管形成的早期阶段。血管萌发来自神经周围血管丛(PNVP)，侵入10日龄胚胎的脐带。它们最常通过背根和腹根之间的外侧表面进入脊髓，较少通过腹侧和/或背侧表面进入，并与通过外侧表面进入的芽吻合。在髓内血管发育过程中，复盖脊髓神经实质的基底板和神经组织与血管壁之间的关系都发生了本质的变化。发育中脊髓的基底板分为三类。第一层是神经周围、外部或初级神经基底板(PNBL)，它是三种信息中最早的一种，覆盖了脊髓的整个外表面。第二种是内部或次级神经基底板(INBL)，它包裹着面对入侵血管壁的神经组织的内表面。第三种类型是围绕血管壁的血管周围基底板(PVBL)。血管通过穿透PNBL进入脊髓。由于PVBL和INBL在血管形成的早期阶段缺乏或不完整，神经组织与髓内血管直接接触。然而，随着它们的发育，形成了边界膜，将神经组织与邻近的血管分开，这是成熟中枢神经系统中毛细血管的特征。血管周围间隙在血管发育过程中可见，继而与髓外结缔组织间隙相连通。它们既不是伪影，也不是髓外结缔组织间隙沿侵袭性萌芽的髓内延伸。边界膜由膜斑块连接或INBL与PVBL融合而成。
The early stages of vascularizations of the spinal cord of the mouse were studied by graphic reconstruction techniques and electron microscopy. Vascular sprouts arise from the perineural vascular plexus (PNVP) to invade the cord of 10-day embryos. These enter the cord most frequently via the lateral surface between the dorsal root and the ventral root; less frequently, they enter via the ventral and/or dorsal surfaces and anastomoses with sprouts that have entered via the lateral surface. During the developement of intramedullary blood vessels there are essential changes both in the basal laminae covering the neural parenchyma of the cord and in the relationship between the neural tissue and vascular walls. The basal laminae of the developing spinal cord were classified into three categories. The first is the perineural, external, or primary neural, basal lamina (PNBL), which is the earliest of the three in formation and covers the entire external surface of the cord. The second one is the internal, or secondary neural, basal lamina (INBL), which invests the internal surface of the neural tissue facing the wall of invading blood vessels. The third type is the perivascular basal lamina (PVBL), which surrounds the vascular wall. Blood vessels enter the spinal cord by penetrating the PNBL. Since the PVBL and INBL are absent or incomplete in early stages of vascularization, the neural tissue is in direct contact with intramedullary blood vessels. However, following their development, boundary membranes are formed, separating the neural tissue from neighboring vessels, a situation characteristic of capillaries in the mature CNS. Perivascular spaces are seen along the course of developing vessels and secondarily become continuous with the extramedullary connective tissue space. They are neither artifact nor intramedullary extensions of extramedullary connective tissue space along invading sprouts. The boundary membranes are formed by connection of membrane plaques or by fusion of the INBL and PVBL.