Metabolomics of Small Numbers of Cells: Metabolomic Profiling of 100, 1000, and 10000 Human Breast Cancer Cells
Metabolomics of Small Numbers of Cells: Metabolomic Profiling of 100, 1000, and 10000 Human Breast Cancer Cells
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DOI：
10.1021/acs.analchem.7b03100
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发表时间：
2017-11-07
影响因子：
7.4
通讯作者：
Li, Liang
中科院分区：
文献类型：
--
作者：
Luo, Xian;Li, Liang
在细胞代谢组学中，需要使用少量细胞进行代谢组学分析，以节省时间和成本。在某些应用中（例如，处理血液中的循环肿瘤细胞），只有有限数量的细胞可用于分析。在本报告中，我们描述了一种基于高性能化学同位素标记 (CIL) 纳流液相色谱质谱 (nanoLC-MS) 的方法，用于对少量细胞（即，
In cellular metabolomics, it is desirable to carry out metabolomic profiling using a small number of cells in order to save time and cost. In some applications (e.g., working with circulating tumor cells in blood), only a limited number of cells are available for analysis. In this report, we describe a method based on high-performance chemical isotope labeling (CIL) nanoflow liquid chromatography mass spectrometry (nanoLC-MS) for high-coverage metabolomic analysis of small numbers of cells (i.e.,