Principles for the Use of In Vivo Transgene Techniques: Overview and an Introductory Practical Guide for the Selection of Tetracycline-Controlled Transgenic Mice
Principles for the Use of In Vivo Transgene Techniques: Overview and an Introductory Practical Guide for the Selection of Tetracycline-Controlled Transgenic Mice
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DOI：
10.1007/978-1-4939-0404-4_4
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发表时间：
2014-01-01
期刊：
影响因子：
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通讯作者：
Sakai, Norio
中科院分区：
文献类型：
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作者：
Sakai, Norio
转基因小鼠是一个有益的工具，可以让研究人员调查特定基因在生理和疾病中的作用。然而，传统的转基因小鼠存在着缺陷，即从胚胎阶段开始的转基因的结构性表达可能会影响小鼠的正常发育或产生代偿效应。为了克服这些缺点，四环素控制的转基因小鼠被开发出来，它可以以组织特异性和时间依赖的方式表达靶基因产物。在这一部分中，首先讨论了四环素控制系统的原理。此外，还介绍了利用该系统产生转基因小鼠的有用信息。
Transgenic mice are a beneficial tool that can allow researchers to investigate the roles of specific genes in physiology and disease. However, conventional transgenic mice have the limitation that constitutive expression of a transgene from the embryonic stage may affect the normal development of the mice or cause compensating effects. To overcome these disadvantages, tetracycline-controlled transgenic mice, which can express target gene products in a tissue-specific and time-dependent manner, have been developed. In this section, the principles of tetracycline-controlled systems are discussed first. In addition, useful information for generating transgenic mice using this system is introduced.