Cryo-electron tomography of Chlamydia trachomatis gives a clue to the mechanism of outer membrane changes
Cryo-electron tomography of Chlamydia trachomatis gives a clue to the mechanism of outer membrane changes
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DOI：
10.1093/jmicro/dfp057
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发表时间：
2010-06-01
期刊：
影响因子：
--
通讯作者：
Zhang, Qinfen
中科院分区：
文献类型：
--
作者：
Huang, Zengwei;Chen, Mukai;Zhang, Qinfen
冷冻电子断层扫描重建沙眼衣原体的结构。结果表明，这种方法比传统的切片方法更好地保留了结构的特征，特别是膜的特征。这种方法还使我们能够确定，基本体的外膜厚度几乎是网状体的两倍。我们的观察为外膜变化的机制提供了线索。
Cryo-electron tomography was employed to reconstruct the structure of Chlamydia trachomatis. Results revealed that the features of the structures, especially those of the membranes, were preserved much better than those by conventional ultrathin section methods. This method also enabled us to determine that the thickness of the outer membrane of the elementary bodies is nearly twice of that of the reticulate bodies. Our observations give a clue to the mechanism of outer membrane changes.